海藻色素糖蛋白对肝癌细胞Caspase-3和Bax蛋白表达的影响(英文)

Effects of Seaweed Pigment Glycoprotein on Caspase-3 and Bax Protein Expression of Hepatoma Cells

目的:研究麒麟菜海藻色素糖蛋白(SPG)对肝癌细胞Caspase-3和Bax蛋白表达的影响。方法:将50只皮下接种H22肝癌细胞株的小鼠随机分为5组,每组10只。高、中、低剂量组分别每天经口灌胃给予100、50、10mg/kg的SPG,肿瘤对照组灌胃生理盐水,连续10d。环磷酰胺组隔天腹腔注射环磷酰胺20mg/kg.bw。取肝癌组织用MTT法测定各组肝癌细胞增殖活性,免疫组化法检测各组肝癌组织Caspase-3和Bax蛋白表达水平。结果:高剂量组Caspase-3和Bax蛋白表达率分别为40.20%和38.10%,而肿瘤对照组分别为5.00%和4.68%,差异均有显著性(P<0.05)。高剂量SPG组和肿瘤对照组的肝癌细胞增殖活性分别为0.711±0.028和1.135±0.032,差别有显著性(P<0.05)。结论:SPG可促进肝癌细胞Caspase-3和Bax蛋白表达,诱发肝癌细胞凋亡。

Objective： To investigate the effect of seaweed pigment glycoprotein （SPG） on the expression of Caspase-3 and Bax protein in hepatoma cells. Methods： Fifty mice inoculated with hepatoma H22 cells were divided into five groups, 10 mice per group. The high, medium and low SPG group were administered by gastric perfusion with 100, 50 and 10 mg/kg/day SPG, respectively. The tumor control group was given the same amount of saline daily. The cyclophosphamide group was given 20 mg/kg cyclophosphamide by intraperitoneal injection every other day. The proliferation activity of hepatoma cells was determined by MTT assay. The expression of Caspase-3 and Bax protein were detected by immunohistochemistry method. Results： The expression of Caspase-3 and Bax protein in high-dose group were 40.20 % and 38.10 % , respectively, while those in the tumor control group were 5.00 % and 4.68 % . The differences between two groups were significant （p0.05）. The proliferation activity of high-dose group and tumor control group were 0.711±0.028 and 1.135±0.032.The difference was significant （p0.05）. Conclusion： SPG can promote the expression of Caspase-3 and Bax protein, and induce the apoptosis of hepatoma cells.