神经酰胺对小鼠皮层神经元MTT代谢率的影响

THE EFFECT OF CERAMIDE ON MTT METABOLISM RATE OF MOUSE CORTICAL NEURON

目的探讨神经酰胺（Ｃｅｒ）对小鼠皮层神经元噻唑蓝（ＭＴＴ）代谢率的影响。②方法以原代培养的２６只小鼠大脑皮层神经元为模型，观察Ｃｅｒ不同浓度（０，５０，１００，２００，５００，１０００，２０００，５０００μｍｏｌ／Ｌ），不同的作用时间（４，８，１２，１６，２４，３６ｈ）对其ＭＴＴ代谢率的影响。③结果ＭＴＴ代谢率随着Ｃｅｒ浓度的增加及作用时间的延长明显下降（ｔ＝２．０８～２．１４，Ｐ＜０．０５）。④结论Ｃｅｒ可损伤神经元线粒体功能，从而导致神经元死亡。

Objective\ To explore the effect of ceramide on MTT metabolism rate of mouse cortical neuron.\ Methods The effect of eramide at different concentrations (0,50,100,200,500,1 000,2 000,5 000μmol/L) and different acting times (4,8,12,16,24,36h) on the metabolism rate of MTT in the primary cell culture of 26 mice cortical neuron were studied.\ Results With the increase of ceramide concentration and acting time, the metabolism rate of MTT decreased (t=2.08-2.14,P<0.05). Conclusion\ Ceramide can damage the function of neuron mitochondrion and then lead to the death of neurons.