胆道梗阻后肝脏自由基损伤及粉防己碱保护作用的实验研究

Experimental study on hepatic free radical injury and the protective effects of tetrandrine after biliary obstruction

目的 观察胆道梗阻后肝脏的自由基损伤和粉防己碱（ Ｔｅｔ） 的保护作用。方法 复制大鼠胆道梗阻模型，每日给予 Ｔｅｔ ３０ ｍｇ／ｋｇ 体重灌胃，动态观测肝组织丙二醛（ Ｍ Ｄ Ａ） 和超氧化物歧化酶（ Ｓ Ｏ Ｄ） 含量及血清 Ｔ Ｂｉｌ、 Ａ Ｌ Ｔ、 Ａ Ｌ Ｐ、 Ｇ Ｇ Ｔ 含量。结果 胆道梗阻后，血清 Ｔ Ｂｉｌ、 Ａ Ｌ Ｔ、 Ａ Ｌ Ｐ、 Ｇ Ｇ Ｔ 水平逐渐升高，肝组织 Ｍ Ｄ Ａ 含量逐渐升高， Ｓ Ｏ Ｄ 逐渐减少，各梗阻组与对照组比较， Ｐ＜ ０ ．０５ 。各 Ｔｅｔ 治疗组与同时相梗阻组比较，血清 Ｔ Ｂｉｌ、 Ａ Ｌ Ｔ、 Ａ Ｌ Ｐ、 Ｇ Ｇ Ｔ 水平下降（ Ｐ＜ ０ ．０５） ；肝组织 Ｍ Ｄ Ａ 含量减少（ Ｐ＜０ ．０１） ， Ｓ Ｏ Ｄ 含量升高（ Ｐ＜ ０ ．０５） 。肝组织 Ｍ Ｄ Ａ 含量与血清 Ａ Ｌ Ｔ、 Ａ Ｌ Ｐ 含量变化呈明显正相关，ｒ 值分别为０ ．９４９ 和０ ．８４３（ Ｐ＜ ０ ．０１） 。结论 自由基损伤可能是胆道梗阻导致肝损害的重要机制之一， Ｔｅｔ对胆道梗阻所致肝损害有明显保护作用。

Objective\ To investigate hepatic free radical injury and the protective effects of tetrandrine (Tet) after biliary obstruction.Method\ The Tet was given to the rat once a day by gavage at a dose of 30 mg/kg. The contents of liver malondialdyhyde (MDA) and superoxide dismutase (SOD), the levels of serum T Bil, ALT, ALP and GGT were determined in each group. Results\ After CBO, serum T Bil, ALT, ALP and GGT levels and liver MDA were increased significantly, while SOD decreased markedly, as compared with control group ( P <0.05). As compared with CBO group at the same obstructed phase, serum T Bil, ALT, ALP and GGT levels and liver MDA in Tet treated groups were decreased significantly ( P <0.05), while liver SOD increased ( P <0.05). The liver MDA content was highly positively correlated with serum ALT and ALP levels ( P <0.01).Conclusion\ Liver free radical injury may be one of the important mechanisms of hepatic injury induced by biliary obstruction. Tet can protect liver effectively from the injury after biliary obstruction.\;