胃癌端粒酶活性检测的临床意义

Telomerase activity in human gastric carcinomas

目的 探讨端粒酶在胃癌组织中的活性及其临床意义。方法 应用银染 Ｔ Ｒ Ａ Ｐ 方法检测９４ 例胃癌组织、１２ 例胃腺瘤、９ 例胃溃疡和５８ 例癌旁正常胃粘膜中端粒酶活性。结果 ９４ 例原发胃癌组织中检出端粒酶阳性８１ 例，阳性率为８６ ．２ ％ 。正常粘膜无１ 例检测到端粒酶表达，１２ 例胃腺瘤有１例表达端粒酶阳性，在９ 例胃溃疡组织中有１ 例检测到端粒酶表达，１２ 例胃腺瘤有１ 例表达端粒酶阳性，在９ 例胃溃疡组织中有１ 例检测到阳性端粒酶表达。胃癌与正常粘膜组端粒酶表达率差异有显著性（ Ｐ＜ ０ ．００１） 。端粒酶活性与肿瘤大小、分化程度、浸润深度、淋巴结转移和 Ｔ Ｎ Ｍ 分期无明显相关（ Ｐ＞ ０ ．０５） 。结论 测定胃癌端粒酶活性对胃癌的诊断有一定意义。

Objective\ To study the telomerase expression in gastric carcinomas and the clinical implications. Methods\ Telomerase activity was detected by a non isotopic PCR based TRAP assay (telomeric repeat amplification protocol) in tissue samples from 94 gastric carcinomas, 58 normal tissues, 12 gastric adenomas and 9 gastric ulcer lesions.Results\ None of the normal tissues exhibited telomerase activity. In contrast, 81 out of the 94 patients with cancers were positive for telomerase expression ( P <0.001), 1 of 12 with gastric adenomas and 1 of 9 gastric ulcer also were positive for telomerase expression. The incidence of telomerase activity in gastric carcinoma tissues was not correlated to tumor size, differentiation, tumor invasion in depth, lymph node metastasis or TNM stage ( P >0.05).Conclusion\ Telomerase might be a good diagnostic marker for the diagnosis of gastric carcinoma.\;