胃肠肿瘤CO_2气腹切口种植的对比研究

A comparison of human gastric cancer cell and human colon cancer cell implantation during carbon dioxide pneumoperitoneum in a murine model

目的 进一步探讨肿瘤细胞切口种植的原因。方法 选用人胃癌细胞株 Ｓ Ｇ Ｃ７９０１ 及人肠癌细胞株 Ｌｏ Ｖｏ，注入雄性 Ｓ Ｄ 大鼠腹腔内，气腹机产生腹腔持续性气腹，通过腹腔穿刺管（５ ｍ ｍ） 收集气体至培养瓶中，３７ ℃、体积分数为５ ％ Ｃ Ｏ２ 环境中培养７ 天后，鉴定是否有肿瘤细胞生长，另取腹腔冲洗液作阳性对照。结果 胃癌细胞持续性气腹测试组中，应用１０６ 个／ｍｌ 胃癌细胞，气腹压达３０ｍ ｍ Ｈｇ ，流量为５ Ｌ／ｍｉｎ ，持续６０ 分钟时，７５ ％ 培养瓶中有肿瘤细胞生长。而相同状态下，未见肠癌细胞生长。结论 相同状态下，胃癌细胞较肠癌细胞更易从切口逃逸，且与肿瘤细胞的数量、气腹压力及持续时间呈正相关。

Objective\ In order to understand the possibility of high port site recurrence in laparoscopic surgery.Methods\ Male Spraque Dawle rats received intraperitoneal injections of different amount of LoVo (10 4/ml) & SGC 7901(10 6/ml). Continuous CO\-2 pneumo was established, CO\-2 emanated from a 5 mm trocar site was collected into dishes. Several samples of peritoneal washing served as positive control. All collected dishes were incubated at 37?℃ and 5% CO\-2 concentration for one week. Results\ After one week of incubation, 3 out of 4 dishes which had continuous flows of CO\-2 gas for 60 min at a high speed of insufflation (5 L/min) in 30 mmHg pneumo with gastric cancer cell injection showed tumor growth, and all peritoneal washing samples demonstrated tumor growth. However, other dishes showed no tumor growth. Conclusion\ Gastric cancer cells can be more easier to cause port site implantation than colon cancer cells at the same condition, and the number of tumor cells, pneumoperitoneum pressure and duration may also affect the occurrence of port site implantation.\;