乳腺癌组织FHIT表达及与ER和PR表达的相关性分析

Analysis on the relativity of FHIT protein with estrogen receptor and progesterone receptor in breast cancer

目的探讨脆性组氨酸三联体(fragile histidine triad,FHIT)基因在乳腺癌组织中的表达,及其与临床病理学特征、雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)表达的相关性及FHIT基因在乳腺癌发生发展中的作用,为乳腺癌治疗及判断预后提供实验依据。方法 应用免疫组织化学染色法检测FHIT在乳腺癌组织和相对正常乳腺组织中(癌周>5 cm)的表达,分析其与ER、PR的表达,乳腺癌的直径大小、组织学分级、TNM分期和淋巴结转移等临床病理学特征的相互关系。结果 FHIT在乳腺癌和相对正常乳腺组织中的阳性表达率分别为32.0%和88.0%,两者有统计学差异(P<0.01)。乳腺癌组织中FHIT阳性表达率在伴淋巴结转移组为44.4%(12/27),在无淋巴结转移组为17.4%(4/23),两者有统计学差异(P<0.05);而在肿瘤大小、组织学分级、TNM分期之间比较,无统计学差异(P>0.05)。FHIT与ER一致表达者共31例,占62.0%。通过相关性分析得出FHIT和ER在乳腺癌的表达相关(r=0.307,P<0.05)。FHIT与PR一致表达者共33例,占66.0%。FHIT表达与PR表达相关(r=0.330,P<0.05)。结论 FHIT表达缺失与乳腺癌的发生及雌孕激素失衡存在一定联系,可为乳腺癌的预后判断、高危患者筛选及内分泌治疗提供依据。

Objective To detect expression of FHIT in breast cancer,and to analyze its relationship with clinic-pathological features and estrogen receptor and progesterone receptor,so as to provide some valuable evidence for diagnosis,biotherapy and prognosis for breast carcinoma.Methods Immunohistochemistry were used to detect the expression of FHIT protein in breast carcinoma tissues and relatively normal breast issues,and the relationship of the expression with clinico-pathological features was analysed;the expression of ER and PR in breast carcinoma,and the relationship of the expression with expression of FHIT protein also did.Results FHIT was located mainly at the cytoplasm of cell in breast issues with spot-distribution,the expression ratios of FHIT in breast carcinoma tissues and relatively normal breast issues were 32.0% and 88.0%.In the patients with breast carcinoma,FHIT expression rate was correlated with lymph node metastasis,and did not related with tumor size,histological grade,TNM stage.The expression of FHIT protein was accorded with the expression of ER in 31 patients with breast carcinoma,and with the expression of PR in 33 patients with breast carcinoma.The rank correlation drawn FHIT protein expression with ER and PR expression associated with statistical significance（P〈0.05）.Conclusion There exists correlation between FHIT gene,ER and PR expression in breast carcinoma.The unbalance of estrogen and progesterone receptor is related to the loss of FHIT protein.