HPA与MMP-9在肾癌中的表达及其与肿瘤微血管密度关系的研究

The expressions of HPA and MMP-9 in renal cell carcinoma and their relationship with the density of tumor angiogenesis

目的检测乙酰肝素酶(HPA)与基质金属蛋白酶-9(MMP-9)在肾癌组织中的表达,探讨它们在肾癌发生发展中的临床意义。方法采用免疫组化SP法检测40例肾癌、10例正常肾组织中HPA与MMP-9及CD34的表达,并记数CD34标记的肿瘤微血管密度(MVD)的表达情况。结果肾癌组织中HPA与MMP-9及CD34的表达高于正常肾组织,HPA与MMP-9两者在肾癌中表达水平成正相关(r=0.352,P<0.05),其中淋巴结转移组HPA与MMP-9的表达与淋巴结未转移组间差异有统计学意义(P<0.05)。HPA与MMP-9的表达在高分化癌、中分化癌和低分化癌中的差别有统计学意义(P<0.05)。HPA与MMP-9在肾癌细胞中的表达与患者年龄、性别无关(P>0.05)。结论 HPA与MMP-9的表达与肾癌的发生、发展、生物学行为及预后密切相关,联合检测HPA和MMP-9的表达水平对于评估肾癌预后有重要意义。

Objective To determine the expressions and significance of heparanase（HPA） and matrix metalloproteinase-9（MMP-9） in renal cell carcinoma.Methods The expressions of HPA,MMP-9 and CD34 in 40 cases of renal cell carcinoma and 10 normal kidney tissues were detected with immunohistochemistry-SP.Results The expressions of HPA,MMP-9 and CD34 were higher in renal cell carcinoma than in the normal kidney tissues.The expressions of HPA and MMP-9 were positively correlated（r=0.352,P 0.05） in renal cell carcinoma.The expressions of HPA and MMP-9 in cases with and without lymph node metastasis were significantly different（P 0.05）.HPA and MMP-9 were differently expressed in well-differentiated cancer,moderately differentiated carcinoma and poorly differentiated carcinoma（P 0.05）.The expression of HPA and MMP-9 in renal cell carcinoma had no relation with the patients＇ age,gender（P 0.05）.Conclusions The expressions of HPA and MMP-9 are closely related with the genesis,development,biological behavior and prognosis of renal cell carcinoma,the detection of which can serve as important markers for the early diagnosis and prognosis of renal cell carcinoma.