期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

微小RNA在膀胱肿瘤组织的表达及其临床意义 被引量：2

Up-regulative expression of microRNAs cluster in human bladder tumor
下载PDF
导出
摘要 目的研究微小RNA(MicroRNAs/miRNAs)microRNAs在人类膀胱肿瘤组织中的表达及其作用。方法取安徽医科大学附属省立医院2009年手术切除的45例膀胱肿瘤组织标本及其肿瘤旁正常组织标本。通过microRNAs微阵点杂交法检测肿瘤组织及其癌旁正常组织的手术标本中microRNAs的表达情况,分析癌组织及其癌旁正常膀胱组织进行定性判断(癌和非癌)。结果人类膀胱肿瘤组织与正常膀胱组织相比,miR-223、miR-26b、miR-221、miR-103-1、miR-185、miR-23b、miR-203、miR-17-5p、miR-23a和miR-205表达显著上调(P<0.005)。结论 miRNAs与膀胱肿瘤的发生发展有密切的联系,有可能成为一个有效的肿瘤标志物以进行膀胱肿瘤的早期预测和诊断。 Objectives Investigate the features of expression of miRNAs in bladder cancer and normal tissues and the significance in the diagnosis of bladder cancer.Methods Tissues of 45 bladder tumors operated in Anhui Provincial Hospital of Anhui Medical University were studied,with adjacent normal tissues as controls.The expressions of microRNAs in the tumor tissues and normal tissues were detected with point by microRNAs microarray hybridization.Results Human microRNAs,miR-223,miR-26b,miR-221,miR-103-1,miR-185,miR-23b,miR-203,miR-17-5p,miR-23a,and miR-205 were significantly up-regulated in bladder cancer（P0.05）.Conclusions MicroRNAs are closely linked to the progression of bladder cancer,and they may serve as effective markers for the early prediction and diagnosis of bladder cancer.
作者 田超 吴奎 曹正国 张旗 宋洪斌 黄昆
机构地区 安徽医科大学附属安徽省立医院泌尿外科 广东省粤北人民医院泌尿外科
出处 《现代泌尿外科杂志》 CAS 2011年第3期262-265,共4页 Journal of Modern Urology
关键词 微小RNA 膀胱癌 肿瘤标记 微阵点 microRNAs bladder cancer Tumor marker microarray
分类号 R737 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1HE L,HANNON GJ. MicroRNAs:small RNAs with a big role in gene regulation[J]. Nat Rev Gene, 2004,5(8) : 522-531.
  • 2BARTEL DP. MicroRNAs: Genomics, biogenesis, mechanism, and function[J]. Cell, 2004 ,116(2) :281-297.
  • 3CHENG A M, BYROM M W. Antisense inhibition of human miRNAand indications for an involvement of miRNA in cell growth and apoptosis[J]. Nucleic Acids esearch, 2005,33 (4) : 1290-1297.
  • 4CALIN GA, CROCE CM. MicroRNA signatures in human cancers[J]. Nat Rev Cancer,2006,6(11) : 857-866.
  • 5LEWIS BP, BURGE CB, BARTEL DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005 , 120 (1) : 15-20.
  • 6GREGORY RI, SHIEKHATFAR R.MicroRNA biogenesis and Cancer [J]. Methods Mol Biol Cancer Res, 2005,65(9):3509-3512.
  • 7ZHANG L, HUANG J, YANG N, et al. microRNAs exhibit high frequency genomic alterations in human cancer[J]. Proc Natl Acad Sci USA, 2G06 ,103(24):9136-9141.
  • 8IORIO MV, FERRACIN M, LIU CG, et al. MicroRNA gene expression deregulation in human breast cancer[J]. Cancer Res, 2005 , 65 (16) ,7065-7070.
  • 9CIAFRE SA, GALARDI S, MANGIOLA A, et al. Extensive modulation of a set of microRnAs in primary glioblastoma[J]. Biochem Biophys Res Commun., 2005 ,334(4) :1351-1358.
  • 10VOLINIA S, CALIN GA, LIU CG, et al. A microRNA expression signature of human solid tumors de? nes cancer gene targets[J]. Proc Natl Acad Sci USA, 2006 , 103 (7): 2257-2261.

同被引文献15

  • 1BARTELI DP. MicroRNAs: target recognition and regulatory functions[J]. Cell, 2009, 136(2):215-233.
  • 2KROL J, LOEDIGE I, FILIPOWICZ W. The widespread regulation of microRNA biogenesis, function and decay[J]. Nat Rev Genet, 2010, 11(9):597-610.
  • 3ZHANG L, HUANG J, YANG N, et al. microRNAs exhibit high frequency genomic alterations in human cancer[J]. Proc Natl Acad Sci USA, 2006, 103(24):9136-9141.
  • 4TAKAMOCHI K, OGURA T, SUZUKI K, et al. Loss of heterozygosity on chromosomes 9q and 16p in atypical adenomatous hyperplasia concomitant with adenocarcinoma of the lung[J]. Am J Pathol, 2001, 159(5):1941-1948.
  • 5TAVAZOIE SF, ALARCON C, OSKARSSON T, et al. Endogenous human microRNAs that suppress breast cancer metastasis[J]. Nature, 2008, 451(7175):147-152.
  • 6MIYAKE H, HARA I, YAMANAKA K, et al. Elevation of serum level of vascular endothelial growth factor as a new predictor of recurrence and disease progression in patients with superficial urothelial cancer[J]. Urology, 1999, 53(2): 302-307.
  • 7MA L, YOUNG J, PRABHALA H, et al. miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis[J]. Nat Cell Biol, 2010, 12(3):247-256.
  • 8DEWS M, HOMAYOUNI A, YU D, et al. Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster[J]. Nat Genet, 2006, 38(9):1060-1065.
  • 9LIU B, PENG XC, ZHENG XL, et al. MiR-126 restoration down-regulate VEGF and inhibit the growth of lung cancer cell lines in vitro and in vivo[J]. Lung Cancer, 2009, 66(2):169-175.
  • 10FISH JE, SANTORO MM, MORTON SU, et al. miR-126 regulates angiogenic signaling and vascular integrity[J]. Dev Cell, 2008, 15(2):272-284.

引证文献2

二级引证文献7

现代泌尿外科杂志
2011年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部