唐可肽对糖尿病大鼠血脂及脂质过氧化作用的影响

The effects of Tangketai on lipid level and lipid peroxidation in diabetic rats

目的探讨唐可肽对糖尿病大鼠脂代谢及脂质过氧化作用的影响。方法用高糖高脂饲料及小剂量链脲佐菌素(streptozotocin,STZ)建立糖尿病大鼠模型,并将成模大鼠分为唐可肽高、中、低剂量组和模型组,同时采用正常的SD大鼠为对照组。唐可肽干预8周后检测各组大鼠的血脂、抗氧化酶和过氧化产物水平,观察肝脏组织的形态学变化。结果与模型组相比唐可肽各剂量组能明显减低糖尿病大鼠血清甘油三酯(triglyceride,TG)、游离脂肪酸(free fatty acids,FFA)和丙二醛(malondialdehyde,MDA)的含量(P<0.05);中、高剂量组能明显降低糖尿病大鼠血清总胆固醇(total cholesterol,TC)的含量,提高超氧化物歧化酶(superoxide diamutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活力(P<0.05);高剂量组能升高高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)而降低低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)的水平(P<0.05)。唐可肽高、中、低剂量组对糖尿病大鼠肝脏组织的脂肪变性有不同程度的缓解作用。结论唐可肽可调节糖尿病大鼠脂代谢紊乱,提高其抗氧化能力,改善其肝脏组织的脂肪变性。

Objective To observe the effects of Tangketai on lipid level and lipid peroxidation in diabetic rats.Methods Diabetic rat model was duplicated with fat-and sugar-rich diet and streptozotocin.Totally 37 rats with diabetes were divided into 4 groups,the diabetic model group and the low-,medium-and high-dose treatment groups.In addition,other normal SD rats served as controls.The diabetic rats in the treatment groups had been fed with Tangketai for eight weeks,and then had their levels of serum lipids,antioxidant enzymes and peroxidative products determined.The morphological changes in their livers were observed.Results The serum levels of triglyceride（TG）,free fatty acids（FFA） and malondialdehyde（MDA） were significantly lower in the treatment groups than in the diabetic model group（P〈0.05）.Compared with the diabetic model group,there was a significant increase in serum superoxide diamutase（SOD） and glutathione peroxidase（GSH-Px） and a significant decrease in serum total cholesterol（TC） in the medium-and high-dose treatment groups（P〈0.05）.Low-density lipoprotein cholesterol（LDL-C） significantly decreased and high-density lipoprotein cholesterol（HDL-C） significantly increased in the high-dose treatment group compared with those in the diabetic model group（P〈0.05）.Tangketai of different dosage had relieving effects of different degree on liver fatty degeneration of the diabetic rats.Conclusion Tangketai can regulate lipid metabolism,enhance the capability of anti-lipid peroxidation and improve liver steatosis of diabetic rats