三叶因子3在胃溃疡大鼠脾脏不同部位的表达

Expression of TFF3 in different positions of the spleen during gastric ulcer in rats

目的研究三叶因子3(TFF3)在胃溃疡大鼠脾脏不同部位的表达。方法胃前壁黏膜下注射冰乙酸制备大鼠胃溃疡模型,免疫组织化学法分别检测正常组(6只)和溃疡组(42只)雄性大鼠脾脏TFF3的表达。结果①溃疡组大鼠脾脏明显肿大、充血;与正常组相比白髓面积明显增大(P<0.05或P<0.01),至6 d组最大,边缘区增厚(P<0.05或P<0.01),至10 d组最厚;红髓面积减少。②TFF3阳性反应物呈棕黄色颗粒状分布于胞质中。③TFF3在正常组大鼠脾脏中呈弱阳性表达,在溃疡组表达明显增高(P<0.05或P<0.01)。溃疡组白髓中阳性细胞TFF3平均吸光度值各组与正常组相比明显增高(P<0.05),以10 d组增高最为明显;边缘区阳性细胞TFF3吸光度值1、2 d和6 d组与正常组相比明显增高(P<0.05或P<0.01),以6 d组增高最为明显;红髓阳性细胞TFF3吸光度值1、2、6 d和14 d组与正常组相比明显增高(P<0.05或P<0.01),以6 d组增高最为明显。④溃疡组边缘区TFF3表达与损伤时间呈负相关(P<0.05)。结论 TFF3在胃溃疡大鼠脾脏各部位呈高水平表达,可能通过提高脾脏免疫反应性促进胃溃疡的愈合。

Objective To study the expression of trefoil factor 3（TFF3） in different positions of the spleen during gastric ulcer in rats.Methods Acetic acid-induced ulcer was induced by injecting acetic acid into submucosa of gastric anterior wall.The expression of TFF3 in the spleen of 6 normal rats and 42 rats with gastric ulcer was detected by immunohistochemistry.Results ① In gastric ulcer rats,the spleen was swollen and congested,and the area of white pulp（WP） was enlarged obviously（P〈0.05 or P〈0.01）,which was the largest in the 6th-day group;the marginal zone（MZ） was thickened（P〈0.05 or P〈0.01）,which was the thickest in the 10th-day group,but the area of red pulp（RP） was reduced.② The positive products of TFF3 were brown and yellow granules distributed in the cytoplasm.③ The expression of TFF3 in the spleen was weakly positive in normal group,but was obviously increased in gastric ulcer group（P〈0.05 or P〈0.01）.The TFF3 average optical density（AOD） values in WP were obviously higher in each gastric ulcer group after gastric ulcer than in normal group（P〈0.05）,which was the highest in the 10th-day group.The TFF3 AOD values in MZ were obviously higher in the 1st-day,2nd-day and 6th-day groups than in normal group（P〈0.05 or P〈0.01）,which was the highest in the 6th-day group.The TFF3 AOD values in RP were obviously higher in the 1st-day,2nd-day,6th-day and 14th-day groups than in normal group（P〈0.05 or P〈0.01）,which was the highest in the 6th-day group.④ The expression of TFF3 in MZ of gastric ulcer group had a negative correlation with injury time（P〈0.05）.Conclusion TFF3 is highly expressed in different regions of the spleen during gastric ulcer in rats.Healing of gastric ulcer may be promoted by enhancing spleen immunoreactivity