联合应用Ag85A和GM-CSF DNA疫苗对小鼠移植性膀胱癌BTT739细胞瘤免疫治疗的研究

The immunological therapy effect of combined Ag85A and GM-CSF DNA vaccine in bladder tumor-bearing mice cells

目的:探讨联合应用Ag85A与GM-CSF DNA疫苗对小鼠移植性膀胱癌BTT739细胞瘤免疫治疗的效果。方法:选用小鼠膀胱癌BTT739细胞株,构建T739小鼠移植瘤模型。随机数字表法分为5组:生理盐水组、空载体组、Ag85A组、联合应用组和BCG组。荷瘤后第7天、14天和21天各肌注1次,末次注射后第7天检测各组小鼠脾脏的CD4+和CD8+T细胞亚群数量及CD4+/CD8+比值、血清IFN-γ和肿瘤重量。结果:与生理盐水组对比,单独应用Ag85A和联合应用Ag85A与GM-CSF DNA疫苗均能够升高CD4+和CD8+T细胞的百分比及CD4+/CD8+的比值,提高血清IFN-γ浓度,降低移植瘤重量,差异具有统计学意义(P<0.05)。联合应用的效果要明显优于单独应用组,差异具有统计学意义(P<0.05),但尚不及单纯应用BCG的免疫治疗效果。结论:联合应用Ag85A与GM-CSF DNA疫苗能够提高荷瘤小鼠的免疫功能,从而达到了抑制肿瘤生长的作用,其效果明显优于单独应用Ag85A DNA疫苗,二者联用具有协同增强免疫效应。

Objective：To explore the immunological therapeutic effect of combined Ag85A and GM-CSF DNA vaccine in bladder tumor-bearing mice cells.Methords： T739 mice with bladder cancer were constructed by injecting mice bladder cancer cell strain BTT739 subcutaneously.The mice were divided randomly into five groups ：NS,pcDNA3.1,Ag85A,Ag85A＋GM-CSF and BCG.DNA vaccines were injected subcutaneously the 7th day,the 14th day,and the 21th day respectively.After 7 days,the number of CD4＋ and CD8＋ T cell subsets was detected,the IFN-γ in mice serum was assayed.At last,the tumor was weighed.Results： Compared to NS group,Ag85A group and Ag85A＋GM-CSF group can upgrade the number of CD4＋ and CD8＋ T cell subsets,the ratio of CD4＋/CD8＋ and IFN-γ level of mice serum,and decrease the weight of tumor（P0.05）.The therapeutic effect of Ag85A＋GM-CSF group was much better than Ag85A group（P0.05）,but still lower than that of BCG group.Conclusion： Ag85A and GM-CSF combined DNA vaccine can upgrade the cellular immunity to inhibit the growth of bladder cancer.Their effects are much better than Ag85A DNA vaccine alone,but still lower than BCG.