黄芪对肺腺癌细胞凋亡及Bcl-2、Bax表达的影响

The influence of Astragalus on apoptosis and Bcl-2/Bax expression of lung adenocarcinoma cells

目的:探讨中药提取物黄芪(Astragalus)对人肺腺癌(lung adenocarcinoma)细胞系SPC-A-1的凋亡诱导作用及其发生机制。方法:体外培养SPC-A-1细胞,用不同浓度黄芪对体外培养的SPC-A-1细胞进行干预。分别采用MTT、免疫细胞化学染色、流式细胞仪法检测其对SPC-A-1细胞的增殖抑制和凋亡诱导作用;并对凋亡相关Bcl-2与Bax蛋白表达的变化进行定性检测。结果:经不同浓度黄芪处理后的SPC-A-1细胞,其生长受到明显的抑制,细胞凋亡指数随药物浓度增加而明显增加;黄芪作用Bax表达明显高于对照组。结论:一定浓度的黄芪能抑制SPC-A-1细胞的增殖,促进其凋亡;其机制可能与调控Bcl-2、Bax表达有关。

Objective：To investigate the effects of Astragalus with active component of Chinese traditional medicine on the apoptosis of human lung adenocarcinoma cell line.Methods： Different concentrations of Astragalus were used in the culture of SPC-A-1 cells.Proliferation and apoptosis of SPC-A-1 cells were observed by MTT,immunocytochemistry,indirect immunofluorescene and flow cytometry quantitative analysis.The expression of Bcl-2 and Bax protein was determined by qualitative analysis.Results： Proliferation of SPC-A-1 cells was inhibited effectively after being treated by different concentrations of Astragalus.The rate of apoptosis was increasing with increased concentration of Astragalus.The expression of Bcl-2 decreased in the experimental groups.Otherwise,the expression of Bax protein was significantly higher than the control group.Conclusion： Astragalus can inhibit proliferation and promote apoptosis of SPC-A-1 cells effectively,the mechanism may be correlated to the up-regulation of Bax and down-regulation of Bcl-2.