热休克凋亡和冻融裂解人胃癌细胞株抗原负载树突状细胞体外刺激细胞毒T细胞活性比较

Comparison of cytotoxic T lymphocyte activity stimulated in vitro by dendritic cells loaded with heat-shocked and freeze-thawed gastric cancer cell strain

目的:比较热休克凋亡和冻融裂解人胃癌细胞株抗原负载树突状细胞(dendritic cell,DC)体外刺激细胞毒T细胞(cytotoxic Tlymphocyte,CTL)的活性。方法:分别制备热休克诱导凋亡胃癌细胞株和肿瘤细胞冻融裂解物,同时分离12例胃癌患者外周血单核细胞体外诱导DC进行负载,并以两种不同方式负载的DC进行淋巴细胞增殖反应及CTL杀伤实验,比较其刺激淋巴细胞增殖及激活的淋巴细胞杀伤靶细胞能力的差异。结果:负载热休克胃癌细胞的DC与负载反复冻融肿瘤细胞裂解物的DC都显示对靶细胞CTL活性,但前者的CTL活性显著高于后者(P<0.05)。结论:用热休克肿瘤细胞负载DC是一个更为有效的负载方式,为临床应用提供了一种可选择的负载方式。

Objective：To compare the cytotoxic T lymphocyte（CTL） activity stimulated in vitro by dendritic cells（DC） loaded with heat-shocked and freeze-thawed gastric cancer cell strain gastric cancer cell strain.Methods： DC isolated from peripheral blood mononuclear cell of 12 gastric cancer peoples were loaded with heat shock-induced apoptotic gastric cancer cell strain and freeze-thawed tumor cell lysates separately.Using lymphocytes stimulated in vitro by DC as effector cells and gastric cancer cell strain as target cells.The cell-killing effect of effector cells on target cells was determined by MTT method.Results： DC loaded with heat shock-induced apoptotic gastric cancer cell strain and freeze-thawed tumor cell lysates both showed the CTL activity on target cells,but the CTL activity of the former was significantly higher than the latter（P0.05）.Conclusion： The method of loading with heat shock-induced apoptotic gastric cancer cell strain was more effective.