神经调节蛋白对小鼠睾丸精原细胞增殖作用的研究

Neuregulin promotes spermatogonia proliferation in mice

目的:研究神经调节蛋白(NRG)对小鼠睾丸精原细胞增殖的影响。方法:将纯化的NRG1β或NRG3的类EGF区域的重组蛋白分别添加到DMEM培养液中,终浓度分别为50、100、200 ng/m l,进行小鼠睾丸断片的器官培养,随后进行B rdU免疫组化染色,检测精原细胞的增殖效应。结果:添加NRG后可以促进精原细胞的增殖活性,与对照组相比具有显著性差异(P<0.05)。按NRG1β50、100、200 ng/m l、NRG3 50、100、200 ng/m l的顺序,精原细胞的增殖活性分别是对照组的1.69、1.55、1.86、1.35、1.54、2.11倍。结论:NRG1β、NRG3能促进小鼠精原细胞的增殖。可以期待NRG应用在男性不育症的治疗上。

Objective： To explore the role of neuregulin（neural regulation of protein,NRG） in the process of mouse spermatogonia proliferation.Methods： Mouse testis fragments were cultured in the medium DMEM containing purified NRG1β or NRG3 at the concentration of 50,100 and 200 ng/ml,respectively,followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.Results： Compared with the control group,neuregulin significantly promoted the proliferation of spermatogonia（P0.05）.The proliferation rates of spermatogonia cultured in the medium with 50,100 and 200 ng/ml of NRG1β were 1.69,1.55 and 1.86 times,and those with 50,100 and 200 ng/ml of NRG3 were 1.35,1.54 and 2.11 times that of the control.Conclusion： NRG1β and NRG3 can promote the proliferation of mouse spermatogonia,and NRG is expected to be applied in the treatment of male infertility.