C57-ras致癌性转基因模型杂交1代的脏器及血液学参数测定

Determination of Hematological and Main Organ Weight Parameters of F1 Carcinogenesis Transgenic C57-ras Mouse Model

目的测定自主建立的致癌性转基因动物模型C57-ras小鼠杂交F1代的血液生理生化值和主要脏器重量,计算脏器系数并作统计学分析。方法选取同窝C57-ras转基因阳性鼠和BALB/cJ小鼠交配后的杂交1代CB6F1-Tg小鼠,雌雄各半,采血,测量血液生理指标和血清生化指标,并称主要脏器重量。结果血液生理指标中,CB6F1-Tg转基因阳性(+/-)雌鼠和阴性(-/-)雌鼠间比较,MCHC存在极显著性差异(P<0.01);CB6F1-Tg转基因阳性(+/-)雄鼠和阴性(-/-)雄鼠间比较,PLT、PCT、EOS%、NEUT、NEUT%、LYM%存在显著性差异(P<0.05),NEUT%、LYM%存在极显著性差异(P<0.01)。血清生化指标中,CB6F1-Tg阳性(+/-)雌鼠和阴性(-/-)雌鼠比较,ALT、TP、ALB存在显著性差异(P<0.05),TG存在极显著性差异(P<0.01)。主要脏器重量和脏器系数均无显著性差异(P>0.05)。结论测定了新建C57-ras致癌性转基因小鼠模型F1代阳性小鼠和阴性小鼠的主要生物学特性,为该模型在致癌性安全性评价等领域的实际应用中提供参考。

Objective To obtain the basic data of biological parameters of F1 of a self-established C57-ras transgenic mice.Methods The blood samples were collected and tested for physiological values using MEK 27222K hematology analyzer and biochemical values using Olympus AU400 auto 2 analyzer.The weight of organs and bodies were determined with electronic balance and the data were analyzed statistically.Results The MCHC of F1 female transgenic mice and non-transgenic mice had extremely significant difference（P〈 0.01）.The PLT,PCT,EOS%,NEUT,NEUT%,LYM% of CB6F1-Tg male mice and non-transgenic mice had significant difference（P〈 0.05）,and that of NEUT%,LYM% had extremely significant difference（P〈 0.01）.Among the sera biochemical data,ALT,TP,ALB had significant difference（P〈 0.05）.The statistical analyzing results of organ weight and coefficient show that had no extremely significant difference（P〈 0.05）.TG had extremely significant difference（P〈 0.01）.Conclusions The F1 of new-established mouse model has been obtained the biological characters with the normal C57BL /6 mice;this may befavorable for its usage in the pre-clinical safety evaluation.