真武汤对阿霉素肾病大鼠治疗作用研究

Pharmacodynamics study on the effect of Zhenwu Decoction on adriamycin nephropathy rat

目的:通过检测阿霉素肾病(adriamycin nephropathy,AN)大鼠的肾比重、血液生化指标及观察肾脏组织病理切片,探讨真武汤对AN大鼠的治疗作用。方法:将大鼠随机分为5组,除正常对照组外,其余大鼠尾静脉注射阿霉素造模大鼠随机分为4组:模型组、缬沙坦组(24mg/kg)、真武汤低剂量组(5.3g生药/kg)、真武汤高剂量组(15.9 g生药/kg),检测其尿蛋白含量。采用HE染色的方法观察肾脏组织形态学变化,检测大鼠的肾比重及0、14、28d大鼠24h尿量及血液生化指标。结果:真武汤5.3g生药/kg可增加AN大鼠24h尿量,真武汤15.9g生药/kg不仅可增加24h尿量而且可以缓解低蛋白血症、降低肾比重、改善肾小球萎缩硬化充血及髓质区蛋白管型等病理变化。结论:真武汤对AN大鼠肾水肿、肾功能下降、肾组织病理性变化具有正相调节作用。

Objective： Investigate the therapeutic effects of zhenwu decoction on adriamycin nephropathy rat by detecting kidney proportion of body weight,blood biochemical index and observing the pathological section of kidney.Methods： Besides A group（normal control group）,the adriamycin nephropathy rats were divided into 4 groups after positive expression of proteinuria detected ： model group,adriamycin nephropathy group treated by valsartan（24mg/kg）,adriamycin nephropathy group treated by zhenwu decoction of low dose（5.3g/kg） and high dose（15.9g/kg）.Detecting the blood biochemical index in 0,14,28 days.All rats were killed after 28 days,measuring the kidney proportion of body weight and observing the pathological section of kidney of HE staining.Result： Low-dose zhenwu decoction can increase the urine volume of 24 hours of AN rats.High-dose zhenwu decoction can not only increase the urine volume of 24 hours but also alleviate hypoproteinemia,decrease the kidney proportion,mitigate atrophy and sclerosis as well as congestion of glomerulus,lessen protein casts of renal medulla.Conclusion： Zhenwu decoction has a protective effect on adriamycin nephropathy rats.