大黄酸对过氧化氢诱导损伤的血管内皮细胞的保护作用

Protective effect of rhein on vascular endothelial cells injured by H_2O_2

目的:观察大黄酸对过氧化氢(H2O2)诱导损伤的血管内皮细胞增殖、凋亡、NO、NOS及SOD表达的影响,探讨大黄酸保护血管内皮细胞氧化损伤的可能机理。方法:100μmol/L H2O2造成血管内皮细胞氧化应激损伤模型,采用倒置显微镜观察细胞形态,Hochest染色观察细胞凋亡情况;MTT法和试剂盒测定各组细胞增殖、NO、NOS的及SOD的表达情况。结果:100μmol/LH2O2可损伤血管内皮细胞、抑制细胞增殖,促进细胞凋亡、降低NO、NOS含量和SOD的活力;而大黄酸则能改善H2O2诱导损伤的血管内皮细胞形态,促进其增殖,抑制其凋亡,提升NO、NOS的含量和SOD的活力,显示大黄酸对血管内皮细胞有一定的保护作用。

Objective： To observe the effect of the cell proliferation,apoptosis,NO,NOS and SOD expression of vascular endothelial cells injured by H2O2,the possible mechanism of rhein against apoptosis was discussed.Methods： Using the microscope and Hochest dyeing to observe the form of cells and apoptosis b;the method of MTT and kit were adopted to determine cell proliferation and expression of NO,NOS and SOD in each group.Result： 100μmol/L H2O2 can injury vascular endothelial cells and inhibit cell proliferation,promote cellular apoptosis and reduce NO,NOS content and SOD vitality;while rhein can improve form of vascular endothelial cell,promote cellular proliferation,restrain cellular apoptosis,advance NO,NOS content and SOD vitality by H2O2.Conclusion： Rhein has a protective effect of vascular endothelial cells.