黄芪皂苷及黄芪甲苷对慢性胃粘膜损伤大鼠胃粘膜上皮细胞超微结构的影响

Astragalosides and astragaloside Ⅳ on gastric mucosal epithelial cells ultrastructural of chronic gastric mucosal injury rats

目的:探讨慢性胃粘膜损伤大鼠胃粘膜上皮细胞超微结构的改变和AST与AST-Ⅳ可能发挥的作用。方法:采用大黄灌胃造成大鼠慢性胃粘膜损伤模型,用扫描电镜观察慢性胃粘膜损伤大鼠胃粘膜表面上皮细胞超微结构的变化及AST与AST-Ⅳ预防给药和治疗给药对其的影响。结果:慢性胃粘膜损伤大鼠胃粘膜上皮细胞广泛坏死、破碎、形态模糊不完整,排列紊乱,细胞肿胀、扁平,细胞间无界限,多个细胞挤压扭结成团;细胞表面微绒毛稀少、脱落,分泌颗粒稀少;胃小凹结构畸形的。AST高(200mg/kg)、低(100mg/kg)剂量及AST-Ⅳ高剂量(4.08mg/kg),预防给药和治疗给药,均能够扭转慢性胃粘膜损伤大鼠胃粘膜上皮的病变,且AST各剂量的作用均优于AST-Ⅳ各剂量,AST高剂量预防给药和治疗给药的作用均明显优于其余各剂量,与正常组的水平相当;AST低剂量预防给药和治疗给药的作用均优于AST-Ⅳ高剂量。结论:慢性胃粘膜损伤大鼠的胃粘膜屏障受到破坏,AST及AST-Ⅳ均具有保护胃粘膜屏障的作用。AST的作用明显优于AST-Ⅳ,且随剂量的增加,AST的优越性更加明显,大剂量的作用接近正常组的水平。AST在较低剂量的情况下可以达到甚至超过AST-Ⅳ大剂量的作用效应。

Objective： To approach the gastric mucosal epithelial cells ultrastructural changes on chronic gastric mucosal injury in rats and the possible role of AST and AST-Ⅳ should play.Methods： The model rats with chronic gastric mucosal injury was made by feeding rheum,then we observed the Ultrastructural changes of gastric mucosal surface epithelial cells and the effects of AST and AST-Ⅳ prevention medication and therapy medication on these model rats by scanning electron microscope.Results： The gastric mucosal epithelial cells of chronic gastric mucosal injury rats became generally necrosis,fragmentation,fuzzy and incomplete form,disorganized arrangement,swelling and applanation,no delimitation among cells,a number of cells squeeze and tangle into lump.Microvillus of cell surface became rare and detached with few secretory granules.The construction gastric pits became malformed.AST high（200mg/kg） and low（100mg/kg） doses as well as AST-Ⅳ high dose（4.08mg/kg）,prevention and therapy medication,were able to reverse the gastric mucosal epithelial lesions in rats with chronic gastric mucosal injury,the effect of AST each dose was superior to all doses of AST-Ⅳ,and the effect of AST high dose of prevention and therapy medication was superior to the rest doses and equal to the normal group.The effect of AST low dose of prevention and therapy medication was superior to AST-Ⅳ high dose.Conclusion： Gastric mucosal barrier of chronic gastric mucosal injury rats get damaged,AST and AST-Ⅳ have a protective effect on gastric mucosal barrier.The effect of AST is obviously superior to AST-Ⅳ,and with the dosage＇s increasing,the superiority of AST is more obviously,the protective effect of AST high dose has already access to the normal group.AST in the case of lower doses can achieve even more than AST-Ⅳ high dose effect.