毛茛总苷对麻醉大鼠血流动力学的影响

Effects of total glycosides total glycosides of Ranunculus japonicus (TGRJ) on the hemodynamics in anesthetic rats

目的:研究毛茛总苷对麻醉大鼠血流动力学的影响。方法:将SD大鼠随机分为5组:生理盐水组、阳性对照组(维拉帕米,10 mg/kg)、毛茛总苷3个剂量组(20、40和80 mg/kg)。采用股静脉注射方式给药,股动脉插管、右颈总动脉心室插管和连续记录肢体Ⅱ导联心电图的方法观察药物对大鼠心率(HR)、收缩压(SP)、舒张压(DP)、平均动脉血压(AP)、左心室收缩压(LVSP)、左心室舒张压(LVDP)、左心室终末舒张压(LVEDP)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax)和等容舒张时间室内压下降的时间常数(T)的变化。结果:静脉注射毛茛总苷80 mg/kg和40 mg/kg后,均能显著地减慢大鼠心率,显著降低SP、DP、AP、LVSP、-dp/dt max和T值;对+dp/dt max和LVEDP无显著性影响。结论:毛茛总苷有减慢大鼠心率,降低动脉血压,抑制心肌收缩和舒张功能的作用。

Objective： To study the effects of total glycosides of Ranunculus japonicus（TGRJ） on the hemodynamics in anesthetic rats.Methods：The SD rats were randomly divided into five groups： saline control group（NS）,positive control group（verapamil,10 mg/kg）,three different doses of TGRJ（20,40,80 mg/kg）（n=10 rats for each group）.Changes of hemodynamic parameters including heart rate（HR）,systolic arterial pressure（SP）,diastolic arterial pressure（DP）,average arterial pressure（AP）,left ventricular pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP）,left ventricular pressure development during isovolumetric contraction/relaxation（±dp/dt max） and T were recorded by cannulating femoral artery,right common carotid artery and consecutive electrocardiographic.Results： TGRJ 80 mg/kg and TGRJ 40 mg/kg can significantly decrease HR,SP,DP,AP,LVSP,-dp/dt max and T（P 0.05 or P 0.01） in anesthetized rats,but there is no obvious change in LVEDP and ＋ dp/dt max.Conclusion：TGRJ reduces HR and BP.It has depressive effects on both myocardial contraction and diastole and negative effects on cardiac function in anesthetized rats.