清开灵对东莨菪碱致小鼠学习记忆障碍的作用及脑内NO和NOS的影响

Effects of Qingkailing on learning memory ability and cerebral nitric oxide and nitric oxide synthase in dementia model mice induced by scopolamine

目的:研究清开灵对东莨菪碱致小鼠空间学习记忆障碍的作用及脑内一氧化氮(NO)和一氧化氮合酶(NOS)的影响。方法:东莨菪碱造模的雄性KM小鼠随机分为6组,正常对照组、模型对照组、清开灵20g/kg组、清开灵40g/kg、清开灵80 g/kg组和阳性对照药尼莫地平0.3g/kg组。给予等容积药物或蒸馏水,每日灌胃一次,连续30天。于末次给药后30min,除对照组腹腔注射生理盐水外,其它各组注射东莨菪碱2mg/kg,20min后进行Morris水迷宫测试,测试5天后处死,取其脑组织进行NO和NOS测定。结果:清开灵可明显改善东莨菪碱所致小鼠的空间学习记忆障碍,并可以降低小鼠脑内NO含量和NOS活性。结论:清开灵能明显改善东莨菪碱所致小鼠空间学习记忆障碍,并降低其脑内NO含量及NOS活性。

Objective： To study the effect of Qingkailing in model mice by measuring learning and memory ability and some biochemical indexes in brain tissue.Methods： The Morris water maze was used to observe the learning and memory ability of model mice.Biochemical methods were used to determine the content of NO and the activity of NOS in brain tissue.Results：（1） Qingkailing could significantly improve the memory of model mice（2） Compared with the control group,Qingkailing could decrease the activities of NOS and the content of NO in the brain tissue obviously.Conclusion： Qingkailing could improve the ability of learning and memory of model mice,and its mechanism might involve in decreasing the activities of NOS and the content of NO in the brain tissue.