滋阴活血方药对糖尿病大鼠牙周组织TNF-α和MMP-3表达的影响

Effect of nourishing yin and promoting blood-flow recipe on TNF-α and MMP-3 expressions in periodontal tissue of diabetes rats

目的:观察糖尿病大鼠牙周组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和基质金属蛋白酶-3(matrixmetalloproteinases-3,MMP-3)的表达,探讨滋阴活血方药(nourishing yin and promoting blood-flow recipe,NYPBR)对二者的影响。方法:选用6月龄雄性Wistar大鼠28只随机分为3组,正常对照组(A组)8只、糖尿病组(B组)和糖尿病中药治疗组(C组)各10只。建立大鼠糖尿病模型,中药治疗组给予NYPBR。给药后12周处死大鼠,取上颌骨标本免疫组化检测牙周组织TNF-α和MMP-3表达并测定其灰度值。结果:B组大鼠牙周组织TNF-α和MMP-3呈强阳性或阳性表达,灰度值明显低于A组(TNF-α:P<0.05,MMP-3:P<0.001)和C组(TNF-α&MMP-3:P<0.001);C组与A组呈弱阳性表达,2组间无明显差异(TNF-α&MMP-3:P>0.05)。结论:过表达的TNF-α和MMP-3可能是造成糖尿病大鼠牙周组织破坏的内在机制之一,NYPBR可使TNF-α和MMP-3表达趋于正常。

Objective： To explore the effect of nourishing yin and promoting blood-flow recipe（NYPBR） on TNF-α and MMP-3 ex- pressions in periodontal tissue of diabetes（MD） rats. Methods： 28 six-month-old male Wistar rats were randomly divided into three groups： 8 heathy rats in the control group（ A）, 10 with DM without treatment in DM group（B） and another 10 with DM and treated by NYPBR in NYPBR group（C）. The DM model was made in group B and C, NYPBR was administered for every rat in group C. After 12 weeks since medicine administration, the rats were killed and the maxilla were collected for detection of TNF-α and MMP-3 expressions by immunohistochemical method. Results： TNF-α and MMP-3 expressions were strongly positive or positive in group B, weakly positive in group C and group A（ P 〉 0.05 ）. The average gray values of TNF-α and MMP-3 in group B were lower than those in group A（ TNF-α：P 〈0.05 ,MMP-3 ：P 〈0.1301 ） and in group C（ P 〈0. 001 ）. Conclusion：Over expressions of TNF-α and MMP-3 might be one of internal mechanisms of periodontal tissue injury caused by DM. NYPBR may down-regulate TNF-α and MMP-3 expressions in DM subjects.