慢性牙周炎患者血清E-selectin的水平及其S128R基因多态性的研究

The serum E-selectin level and the S128R polymorphisms of E-selectin in patients with chronic periodontitis in Chinese population

目的:探讨中国汉族人群中E-选择素第4外显子S128R基因多态性与慢性牙周炎(chronic periodontitis,CP)的相关性,以及CP对血清E选择素水平的影响。方法:采用病例-对照研究方法,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测CP患者和健康对照者的E-选择素第4外显子S128R的基因型和等位基因分布,同时采用酶联免疫吸附试验(ELISA)检测CP患者和对照者血清E-selectin水平。结果:基因型频率符合Hardy-Weinberg平衡。E-selectin基因型SS、SR频率在对照组与CP组分别为93.7%、6.3%和83.9%、16.1%,等位基因S、R频率在对照组和CP组分别为96.9%、3.1%和92.0%、7.97%。基因型频率和等位基因频率在CP组和对照组间的差异均有显著性(P<0.05),CP患者血清E-selectin水平显著高于对照组(P<0.01)。结论:E-选择素第4外显子S128R基因多态性与CP有相关性,R等位基因可能是CP患者易感性的遗传标志之一。CP患者血清E-selectin水平明显升高。

Objective： To study the correlation between E-selectin S128R polymorphism and chronic periodontitis in Chinese population. Methods： The S128R polymorphism in the exon 4 of E-selection was detected by polymerase chain reaction restriction fragment length polymorphism method （PCR-RFLP） in patients with chronic periodontitis （n = 143 ） and in healthy controls （n = 142）. The serum level of E-selectin was determined by enzyme-linked immunosorbent assay （ELISA）. Results： Genotype frequency was accord with the balance of Hardy- Weinberg. In control group the frequency of genotype SS and SR was 93.7% and 6.3%, in CP group that was 83.9% and 16.1% respectively（P =0. 009）. In control group the frequency of allele S and R was 96.9% and 3.1% , in CP group that was 96.9% and 3.1% respectively（ P = 0.012 ）. There were significant difference in frequency of genotype and allele between CP and control groups. The serum levels of soluble E-selectin（txg/L） in the CP group and the control was 47.6 ±6.71 and 29.37±7.28 respectively （P 〈0.01 ）. Conclusion： The S128R polymorphism in the exon 4 of E-selection was related to chronic periodontitis. Allele R may be one of the genetic sign of chronic periodontitis susceptibility. The serum levels of soluble E-selectin in the CP group is obviously higher.