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带TrpC启动子的质粒pYG715/Vgb对顶头孢霉的转化 被引量:4

Transformation of plasmid pYG715/Vgb bearing TrpC promoter in Cephalosporium acremonium
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摘要 分别以来自于构巢曲霉和酿酒酵母的启动子TrpC和TEF1来构建质粒pYG715/TrpC和pZR333并转化头孢菌素C工业生产菌种顶头孢霉,结果发现只有pYG715/TrpC产生转化子。 Cephalosporium acremonium , the producer of cephalosporin C, was transformed with plasmid pYG715/Vgb containing the both of a phleomycin resistance gene and a Vitreoscilla hemoglobin gene, which were cloned downstream of the TrpC gene promoter from Aspergillus nidulans . Analysis of Southern hybridization demonstrated the integration of the transformed DNA into the chromosomal DNA of Cephalosporium acremonium .
出处 《中国抗生素杂志》 CAS CSCD 北大核心 1999年第4期269-272,共4页 Chinese Journal of Antibiotics
基金 国家自然科学基金
关键词 顶头孢霉 启动子 质粒 Β-内酰胺 TRPC 转化 Cephalosporium acremonium Promoter Plasmid
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参考文献3

  • 1Zhang J Y,Arch Microbiol,1992年,158卷,364页
  • 2金冬雁(译),分子克隆实验指南(第2版),1992年,40页
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同被引文献44

  • 1高兴喜,杨谦.PEG-CaCl_2介导苏云金杆菌CryⅠA(b)基因转化哈茨木霉[J].哈尔滨工业大学学报,2005,37(10):1333-1336. 被引量:4
  • 2徐明良,杨金水,高嵩,钱旻,葛扣麟.部分酶解酵母高效电击转化研究[J].微生物学报,1996,36(6):428-432. 被引量:2
  • 3MATHISON L, SOLIDAY C, STEPAN T,et al. Cloning, characterization and use in strain improvement of the Cephalosporium acremonium gene eefG encoding acetyl transferase[J]. Curr Genet, 1993,23 ( 1 ) : 33 -41.
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  • 5Maniatis T, Fritsh E F, Sambrook J. Molecular cloning: A laboratory manual. 2^nd ed. New York: Cold Spring Harbor laboratory Press,1989.
  • 6DeModena J A, Gutierrez S, Velasco J, et al. The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of bacterial hemoglobin. Biotechnology,1993, 11:926 - 929.
  • 7Martin J F. Molecular control of expression of penicillin biosynthesis genes in fungi: regulatory proteins interact with a bidirectional promoter region. J Bacteriol, 2000, 250:367 - 374.
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  • 9Schmitt E K, Kempken R, Koch U. Functional analysis of promoter sequences of cephalosporin C biosynthesis genes from Acremonium chrysogenum: specific DNA-protein interactions and characterization of the transcription factor PACC. Mol Genet Genomics, 2001, 2,65:508 - 518.
  • 10Skatrud P L, Queener S W, Carr L G, et al. Efficient integrative transformation of Cephaloporium acremonium. Curt Genet, 1987,12:337 - 348.

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