毛细管胶束电动色谱法分离和定量5种喹诺酮类药物

Separation and determination of five quinolones by micellar electrokinetic capillary chromatography

建立了５种喹诺酮类药物的毛细管胶束电动色谱分离和尿样品的定量测定方法。测定条件为：运行缓冲液：５０ｍｍｏｌ／Ｌ磷酸二氢钠－１００ｍｍｏｌ／Ｌ溴化十六烷基三甲胺（１∶１，ｐＨ４．５）；操作电压１２ＫＶ，检测波长２８０ｎｍ。２０ｍｉｎ内５种喹诺酮药物全部得到分离，并与毛细管区带电泳作了比较。尿液对样品的测定无干扰。线性范围为５．０～３５．０ｍｇ／Ｌ，最低检测浓度为２ｍｇ／Ｌ，ＲＳＤ小于１０％。

A method has been developed for the separation and determination of quinolones (pipemidic acid, lomefloxacin, levofloxacin, ciprofloxacin and norfloxacin) in human urine by micellar electrokinetic capillary chromatography (MEKC). Measurements were carried out in a buffer solution containing 25 mmol/L sodium dihydrogen phosphate and 50 mmol/L cetyltrimethylammonium bromide (CTAB), (pH4.5), at 12KV running voltage applied to a 50cm×50μm i.d. (45.5 cm effective length) capillary. Selectivity in the separation of five quinolones was improved by using MEKC in comparision with capillary zone electrophoresis (CZE). By MEKC, we determinated the concentrations of five quinolones in human urine. The recovery ranged from 85.07% to 113.70% with RSD less than 10%. The linear ranges were 5.0￣35.0mg/ml.