贵州南五味子多糖对鸡细胞免疫和体液免疫的影响

Effects of Guizhou Kadsura polysaccharide(GKP) on cellular and humoral immunity of chicken

选用600只15日龄海兰褐雏鸡,随机分为5个处理,每个处理3个重复,每个重复40只鸡,皮下注射贵州南五味子多糖10、20mg/kg、黄芪多糖20mg/kg、环磷酰胺40mg/kg及生理盐水。空白对照组,连续给药3d后免疫新城疫疫苗,分别于免疫后7、14、21、28和35d时,各处理组随机抽取8只鸡检测各项免疫指标,探讨不同处理组对雏鸡免疫功能的影响。本试验结果表明,皮下注射10～20mg/kg贵州南五味子多糖可显著促进鸡外周血T淋巴细胞增殖,IL-2的产生(P<0.01);可使CD4+/CD8+淋巴细胞比值明显升高(P<0.01);可使鸡体内抗ND抗体滴度明显升高(P<0.01);可使法氏囊、胸腺的脏器指数均明显升高(P<0.01)。

The experiment was conducted to investigate the effects of Guizhou Kadsura polysaccharide（GKP） on cellular and humoral immunity of in chickens by subcutaneous injection.A total of 600 Hy-line brown chickens aged 15-day-old were randomly divided into five groups with three replicates of forty chickens each.Experimental chinckens were injected with GKP（20 mg/kg and 10 mg/kg）,APS（20 mg/kg）,cyclophosphamide（40 mg/kg） respectively per day for and normal sodium（0.09%NaCl） respectively per day for three days.Then Experimental chinckens were injected with the same Newcastle disease vaccine at the third day.At 7,14,21,28 and 35 days after vaccination,8 chickens were sampled randomly from each group for determination of the immune parameters.The results showed that GKP（10-20 mg/kg） could increase ConA response of chick peripheral blood lymphocyte（PBL） significantly,and could enhance LPS multiplication.GKP could result in the increased ratio of CD4＋/CD8＋ T lymphocyte,significant increase in antibodies to ND and the production of IL-2,with significant difference to compare with other control group（P0.01）.And GKP（10-20 mg/kg） could result in significant increase in organ index of thymus gland,bursa of Fabricius（P0.01）.