锌、铜对实验性钼中毒绵羊肝脏、肾脏中钼含量的影响

Effect of copper and zinc on the level of molybdenum in liver and kidney of sheep with experimental molybdenum poisoning

小尾寒羊20只,随机分为5组,分别口服钼、铜和锌(mg/kg),Ⅰ组30mg/kg钼、Ⅱ组30mg/kg钼+15mg/kg铜、Ⅲ组30mg/kg钼+15mg/kg锌、Ⅳ组30mg/kg钼+15mg/kg铜+15mg/kg锌,Ⅴ组去离子水。试验90d期满,采取肝脏和肾脏,硫酸-高氯酸消化,原子吸收法测定钼、锌、铜含量,结果显示,Ⅰ组肝脏钼含量与第Ⅱ、Ⅲ、Ⅳ、Ⅴ组差异极显著(P<0.01),Ⅱ、Ⅲ组与Ⅳ、Ⅴ组差异极显著(P<0.01);Ⅳ组肝脏铜含量与Ⅰ、Ⅲ、Ⅴ组差异极显著(P<0.01),与Ⅱ组差异显著(P<0.05),Ⅱ组肝铜含量与Ⅰ、Ⅲ、Ⅴ组差异极显著(P<0.01);组间肝脏锌含量均差异极显著(P<0.01)。Ⅰ、Ⅲ组肾钼含量显著高于Ⅱ、Ⅳ、Ⅴ组(P<0.01);Ⅱ与Ⅳ、Ⅴ组差异极显著(P<0.01);肾铜含量Ⅲ组与Ⅰ组差异极显著(P<0.01);Ⅱ、Ⅲ、Ⅳ组与Ⅰ、Ⅴ组差异极显著(P<0.01);各组肾脏锌含量差异均极显著(P<0.01)。结果表明,30mg的钼可使肝脏钼含量显著增加(P<0.01),锌、铜含量降低。锌对肝脏钼有很好的颉抗作用,铜、锌有效地降低了钼在组织中的蓄积。

Twenty small tail Han sheep were allocated to five groups treated with 30 mg/kg Mo（Ⅰ）,15 mg/kg Cu（Ⅱ）,15 mg/kg Zn（Ⅲ）,30 mg/kg Mo＋15 mg/kg Cu＋15 mg/kg Zn（Ⅳ） as CuSO4·5H2O or ZnSO4·7H2O,and deionized water（Ⅴ）.After 90 days,liver and kidney were collected for detecting the concentration of molybdenum,copper and zinc.Group Ⅰ had higher level of molybdenum in the liver than that of other groups（P0.01）,and group Ⅱ and Ⅲ had higher level of Mo in the liver than that of group Ⅳ and Ⅴ（P0.01）;group Ⅳ had higher level of copper in liver than that of group Ⅰ,Ⅲ,Vs（P0.01）,and higher than group Ⅱs（P0.05）;the difference of zinc between each group was obvious in liver（P0.01）.Group Ⅰ,Ⅲ had higher level of Mo in kidney than group Ⅱ,Ⅳ,Ⅴ（P0.01）,and group Ⅱ was higher than group Ⅳ and Ⅴ（P0.01）;Group Ⅰ and Ⅱ had higher level of copper in kidney than group Ⅱ,Ⅲ,Ⅳ＇s（P0.01）;the difference of zinc between each group was obvious in kidney（P0.01）.Zinc had an accumulation effect in the liver and kidney,copper had an antagonistic effects on molybdenum in liver.