Survivin在活化的肝星状细胞中的表达及其意义

Expression of survivin in active hepatic stellate cells

目的了解survivin在活化的肝星状细胞中的表达及其意义。方法用细胞免疫荧光和RT-PCR方法检测survivin在活化的肝星状细胞中的表达,并以肝癌细胞和肝细胞为对照。结果细胞免疫荧光显示survivin在活化的肝星状细胞中明显表达,阳性率为(66.07±8.55)%,明显高于肝细胞(9.74±2.68)%,两者比较差异有显著性(P<0.05)。而且肝细胞表达荧光强度很弱;在肝癌细胞中表达率最高,阳性率达(69.41%±9.10)%。RT-PCR结果显示survivin mRNA在肝星状细胞和肝癌细胞中检测到,在肝细胞中未检测到。结论 survivin在活化的肝星状细胞中有明显表达,可能与肝星状细胞中抑凋亡基因survivin过度表达有关。因此,抑制活化的肝星状细胞中survivin的表达,可能对抑制活化的肝星状细胞增殖和诱导其凋亡产生效应。

【Objective】 To investigate the expression and significance of survivin in active hepatic stellate cells（HSCS）.【Methods】 The expressions of survivin were detected in actived HSCS by immunofluorescence and RT-PCR,and hepatic carcinoma cells and normal liver cells were as control groups.【Results】 The expressions of survivin in actived HSCS were obviously higher than the those in normal liver cells.The expression rates of them were（66.07±8.55）% and（9.74±2.68）%,respectively.There was statistical difference between actived HSCS and normal liver cells（P 0.05）.The expression rates of hepatic carcinoma cells were the highest in three groups cells（69.41±9.10）%.Survivin mRNA could be detected in HSCS and hepatic carcinoma cells,but it could not be detected in normal liver cells.【Conclusion】 There was higher survivin expression showed in actived HSCS.Therefore,inhibiting the expression of survivin in actived HSCS can suppress the proliferation and promote the apoptosis in actived HSCS.