大鼠抑郁模型的血清细胞因子的动态研究

Dynamic research of the cytokines in serum of rat depressive model

目的探讨大鼠抑郁模型的血清细胞因子IL-2、IL-6及IL-10的动态变化及临床意义。方法 30只SD大鼠根据行为学评分后,随机分为对照组和抑郁组,每组15只。运用慢性不可预知应激及孤养模式建立抑郁模型,应激共8周。于建模开始前及每两周末进行大鼠体重测量、液体消耗实验及Morris水迷宫试验,并用酶联免疫吸附法(ELISA)检测每组大鼠的血清炎症因子IL-2、IL-6及IL-10的变化。结果各项行为学指标表明建模成功并呈动态变化。IL-2在0～4周时缓慢上升,至第4周时,抑郁组较对照组差异有显著性(P<0.05),其后缓慢下降;IL-6在第2周时,抑郁组就明显上升,较对照组差异有显著性(P<0.05),2～4周继续上升(P<0.01),至第6周时下降,但第8周时略上升;IL-10在第2周时,抑郁组略下降,第2～6周时上升,于第6周时较对照组差异有显著性(P<0.05),第8周时保持较高水平。结论大鼠抑郁模型的血清细胞因子IL-2、IL-6及IL-10存在异常,并随着应激的推进呈动态变化。

【Objective】 To explore the dynamic changes and significance of the serum cytokines（IL-2,IL-6,IL-10） of rat depressive model.【Methods】 Based on the evaluation of ethology,fifty SD rats were randomly and equally divided into two groups： the control group and the model group which was exposed to chronic unpredictable mild stress for 8 weeks.The body weight,fluid consumption test and Morris water maze test were measured while the cytokines（IL-2,IL-6,IL-10） in serum were done by ELISA at the beginning and per two weeks.【Results】 Based on the behavioral data,the rat depressive model has been established successfully,changing dynamically.IL-2 of the depressive group increased from the beginning to 4th week when it was significantly higher at 4th week（P 0.05） and then decreased slowly.IL-6 of the depressive group was significantly higher at the second week（P 0.05） and increased from 2nd to 4th week,while decreased at 6th week and increased a litter at 8th week.IL-10 of the depressive group decreased at 2nd week and increased from 4th to 6th week when it＇s significantly higher（P 0.05）,and kept high level at 8th week.【Conclusion】 The serum cytokines（IL-2,IL-6,IL-10） of rat depressive model were abnormal,and changed dynamically with the development of stress.