大肠癌患者癌细胞DNA含量及倍体分布与某些胃肠激素水平的关系

Analysis of the relation between cancer cell DNA and changes of gastrin and somatostatin in patients with colorectal cancer

目的：探讨大肠癌患者癌细胞 Ｄ Ｎ Ａ 含量、倍体分布与体内胃泌素（ Ｇ）、生长抑素（ Ｓ Ｓ）水平变化，以及肿瘤临床病理参数之间的关系。方法：以图像分析法测定４０ 例大肠癌患者癌细胞 Ｄ Ｎ Ａ 含量及二倍体偏离指数（２ｃ Ｄ Ｉ）；放免法测定血清 Ｇ，血浆、肿瘤、癌近旁粘膜和癌远旁粘膜的 Ｓ Ｓ水平；借助计算机以多因素逐步回归法分析癌细胞 Ｄ Ｎ Ａ 含量、２ｃ Ｄ Ｉ、上述胃肠激素水平与肿瘤临床病理特点之间的关系。结果：肿瘤 Ｓ Ｓ上升伴随着 Ｄ Ｎ Ａ 含量和２ｃ Ｄ Ｉ显著下降（ Ｐ＜ ０．０１）；癌近旁粘膜 Ｓ Ｓ与 Ｄ Ｎ Ａ 含量（ Ｐ＜ ０．０５）和２ｃ Ｄ Ｉ（ Ｐ＜ ０．０１）呈显著正相关；直肠癌细胞 Ｄ Ｎ Ａ 含量和２ｃ Ｄ Ｉ明显增高（ Ｐ＜ ０．０１）。结论：内源性 Ｓ Ｓ参与了对癌细胞 Ｄ Ｎ Ａ 合成的调节；提高肿瘤和癌近旁粘膜 Ｓ Ｓ水平将有助于抑制大肠癌细胞内 Ｄ Ｎ Ａ 的合成。

Objective: To evaluate the relation between DNA contents, 2c deviation indexes(2cDI) of colorectal cancer cells,changes of some gastrointestinal hormones levels,and the cancer clinical pathologic features. Methods: In 40 patients with colorectal cancer, DNA contents, 2cDI of the tumor cells (determined by automatical imaging cytometry), serum gastrin, plasma somatostatin(SS),tumor SS, cancer adjacent mucosa SS and cancer distant mucosa SS levels (assayed by RIA), and the cancer clinical pathologic features were analyzed by multiple variable stepwise regression with a computer. Results: The increases of tumor SS closely accompanied by the decrease of the cancer cell DNA contents or 2cDI ( P <0.01). Cancer adjacent mucosa SS had a significant positive correlation to the cancer cell DNA contents or 2cDIs ( P <0.05 and P <0.01, respectively). The cancer cell DNA contents and 2cDIs increased significantly in patients with rectal cancer ( P <0.01). Conclusion: The endogenous SS may play an important role in the regulation of DNA synthesis in cancer cell, elevating the tumor SS level and keeping the cancer adjacent mucosa SS at high level, thus inhibiting the DNA sythesis in colorectal cancer cell.