摘要
构建含有Swollenin基因(swo基因)的香菇表达载体,并通过PEG(polyethylene glyeol)介导的原生质体转化法实现对香菇的遗传转化,转化效率约为1个转化子/μg质粒DNA。PCR和杂交检测结果表明,swo基因已经整合到香菇的基因组中;栽培实验结果表明,两个转化子都能正常出菇,而且转化子Ⅱ表现出优良的生物学性状,将swo基因转化到香菇中对香菇新品种的培育进行探索。
Swollenin can loosen cellulose and enhance its enzymatic hydrolysis.An expression plasmid containing swollenin(swo) gene was constructed and successfully transformed to Lentinus edodes by PEG-mediated transformation method with an efficiency of 1 transformant/μg plasmid DNA.PCR and Southern blot indicated that swollenin was successfully integrated to the genome of L.edodes.Both tested transformants of L.edodes can form fruit bodies and transformant II still revealed good cultivation characteristics.Transformation of swo gene into L.edodes can provide an exploration into breeding of new varieties of L.edodes.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第11期141-145,共5页
Food Science
基金
山东省自然科学基金项目(Q2008D02
ZR2010CL020)
国家现代农业(食用菌)产业技术体系建设专项(nycytx-28)
山东省农业良种工程项目(2010LZ006-04)
2010年山东省农业应用技术重大创新专项资金课题