不同破壁方法提取酵母菌总RNA的比较

Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction

选择液氮碾磨、反复冻融、超声波、加玻璃珠漩涡振荡和蜗牛酶酶解5种方法破碎酵母菌细胞壁,再使用Trizol法提取酵母菌总RNA,通过对总RNA进行质量浓度和纯度分析,比较不同破壁方法对酵母菌总RNA提取的影响。结果表明:在使用Trizol法提取酵母菌总RNA的实验中,反复冻融和液氮碾磨是较为有效且简便的酵母菌细胞壁破碎方法。

Five methods such as liquid nitrogen grinding,repeated freeze-thawing,ultrasonic treatment,vortex shaking with glass beads and snailase hydrolysis were used to break the cell wall of yeast for the extraction of total RNA by the Trizol method.Based on total RNA concentration and purity,these methods were compared for their effects on total RNA extraction from yeast.The results showed liquid nitrogen grinding and repeated freeze-thawing were the most effective and convenient methods for total RNA extraction from yeast by Trizol.