低聚乳果糖对结肠炎大鼠食糜代谢物影响

Effect of Lactosucrose on Chyme Metabolites in Rats with Colitis

目的:建立大鼠溃疡性结肠炎模型,观察低聚乳果糖(LS)对结肠炎大鼠食糜代谢物的影响,并探讨其改善大鼠结肠炎的作用机制。方法:三硝基苯磺酸(TNBS)建立溃疡性结肠炎模型,分为4组:正常组、模型组、LS组、柳氮磺胺吡啶(SASP)药物组。21d后宰杀大鼠,测定盲肠和结肠食糜中短链脂肪酸、pH值和NH3-N含量。结果:TNBS造模后,与正常组相比,大鼠盲肠和结肠食糜中乙酸、丁酸含量显著降低,其中结肠食糜中丙酸的含量极显著降低,pH值和NH3-N的含量显著增高。LS干预后与模型组相比较,盲肠段食糜中乙酸含量显著增加(P<0.05),丁酸含量极显著增加(P<0.01),pH值显著降低(P<0.05)、NH3-N含量极显著降低(P<0.01),各指标均恢复到正常水平;结肠食糜中NH3-N的含量显著降低(P<0.05)。结论:LS可能通过提高肠道中丁酸的含量、降低肠道pH值和NH3-N的含量来缓解对结肠炎大鼠的损伤作用。

Objective： To explore the effect of lactosucrose on hime metabolites in established rat model with ulcerative colitis.Methods： TNBS was used to induce ulcerative colitis in rats,and the obtained ulcerative colitis rats were randomly divided into 4 groups including the normal group,model control（MC） group,lactosucrose（LS） group and sulfasalazine（SASP） group.The last two groups were orally administered 2 mL of normal saline containing 250 mg/kg LS and normal saline containing 250 mg/kg SASP for 21 consecutive days,respectively.The normal and model groups were given the same volume of normal saline.All the mice were killed after 24 hours of fasting after the last administration.The short chain fatty acid contents,pH and NH3-N in cecum and colon were determined.Results： Compared with the NC group,a significantly decreased acetic acid and butyric acid in cecum and colon,a significantly declined propionic acid in colon and a significantly increased pH and NH3-N were observed in MC group（P 0.01）;Compared with MC,when the rats treated with LS,acetic acid（P 0.05） and butyric acid（P 0.01） in cecum were significantly increased;pH（P0.05） and NH3-N（P 0.01） were significantly decreased;NH3-N in colon were significantly decreased（P 0.05）.Conclusion： LS can be used as a functional food with potential therapeutic effect on colitis.The mechanism for relieving the injury of colon tissues in colitis may be due to the increased content of butyric acid and decreased content of NH3-N in the intestinal tract.