姬松茸多糖组分对小鼠巨噬细胞作用研究

Effect of Active Polysaccharide Fraction from Agaricus blazei Murill on Mouse Macrophages

为研究姬松茸多糖组分对小鼠巨噬细胞的激活作用,用姬松茸多糖组分(ABMB3)刺激体外培养的小鼠骨髓来源巨噬细胞,检测刺激后巨噬细胞分泌至培养基中的肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、NO含量以及小鼠巨噬细胞对乳胶颗粒吞噬率的变化,并检测ABMB3刺激的巨噬细胞对肿瘤细胞的抑制作用,结果显示:经姬松茸多糖组分ABMB3刺激后能显著刺激巨噬细胞分泌TNF-α和IL-1β,并产生大量的NO,小鼠巨噬细胞对乳胶颗粒的吞噬功能也明显增强。而且可以通过激活巨噬细胞,增强其吞噬能力而达到抑制肿瘤细胞增殖的功效。

Agaricus blazei Murill（ABM） is rich in polysaccharides,and has obvious treatment and prevention efficacy for cancers.A bioactive polysaccharide fraction（ABMB3） was isolated from hot water extract of A.blazei fruit bodies and purified by ethanol precipitation,anion-exchange and gel filtration chromatography.Phase-contrast microscopic observations revealed that the size of mouse macrophages was increased after exposure to ABMB3.ABMB3-activated macrophages could secret TNF-α and IL-1β,and produce a large amount of NO in vitro.Meanwhile,the capacity of the cells for phagocytizing latex beads was also significantly increased.Moreover,ABMB-3-induced activation of mouse macrophages in the presence of L929 tumor cells for 48 h could result in an increased percentage of phagocytes target cells when compared with untreated controls.Therefore,ABMB3 alone had no effect on the growth of L929 cells.