微生态补钙制剂的生物利用研究

Bioavailability Study of Microbial Calcium Produced by Co-fermentation of Lactobacillus rhamnosus and Lactobacillus acidophilus

补钙制剂的生物利用率是评价补钙效果的重要指标。以KM种健康小鼠为模型,饲喂不同钙源及钙含量的饲料,测定饲料消耗量、粪钙、尿钙、骨钙和血钙,计算钙的表观吸收率及存留率,并与对应剂量的碳酸钙组对比,评价微生态补钙制剂的生物利用情况。结果表明:微生态补钙制剂低剂量组和标准剂量组的骨钙含量与相同水平的碳酸钙对照组比较差异显著(P<0.05),钙的表观吸收率明显高于对应碳酸钙剂量组(P<0.01);微生态补钙制剂组与碳酸钙对照组相比,血钙水平差异不显著(P>0.05),但均高于碳酸钙对照组。表明微生态补钙制剂生物利用率高,是一种理想的补钙制剂。

Bioavailability of calcium preparations is an important indicator for evaluating calcium absorption efficiency.In order to evaluate bioavailability of a microbial calcium from co-cultured Lactobacillus rhamnosus and Lactobacillus acidophilus in bovine bone power medium,KM mice fed feed containing different calcium sources and contents were used as the model to determine feed consumption,fecal calcium,urinary calcium,bone calcium and blood calcium,and calculate the absorption rate and retention rate of calcium.Meanwhile,the bioavailability of the microbial calcium was compared with that of calcium carbonate at identical doses.The results indicated that the bone calcium content in the microbial calcium group at low or standard dose revealed a significant difference when compared with the calcium carbonate agent at the same dose（P 0.05）.An obviously higher apparent absorption rate of the microbial calcium was observed when compared with calcium carbonate group（P 0.01）.No obvious difference in blood calcium level between the microbial calcium group and calcium carbonate group was observed although the blood calcium level was higher in the microbial calcium group（P 0.05）.Therefore,the microbial calcium is an ideal calcium preparation with high biological activity and bioavailability.