杜仲雄花水溶性生物碱的镇静催眠作用

Sedative and Hypnotic Effects of Water-soluble Alkaloids from Male Flowers of Eucommia

目的:研究不同剂量杜仲雄花水溶性生物碱的镇静催眠作用。方法:采用酸水提取法提取杜仲雄花中水溶性生物碱。观察不同剂量杜仲雄花水溶性生物碱对小鼠自主活动的影响以及直接镇静催眠作用、与戊巴比妥钠的协同作用和抗惊厥作用。结果:杜仲雄花水溶性生物碱能有效减少小鼠的自主活动次数;与戊巴比妥钠有较好的协同作用,能显著增加阈下剂量的小鼠睡眠率,延长阈下剂量及阈上剂量的睡眠时间,并缩短睡眠潜伏期;杜仲雄花水溶性生物碱还能有效降低尼可刹米所致的小鼠惊厥率,延长惊厥潜伏期。结论:杜仲雄花水溶性生物碱具有良好的镇静催眠作用。

Objective： To explore the sedative and hypnotic effects of different doses of water-soluble alkaloids from male flowers of Eucommia.Methods： Water-soluble alkaloids were extracted from Eucommia male flowers by an acidic water extraction method,their effect on spontaneous activities of mice,hypnotic function with and without the presence of pentobarbital sodium and anticonvulsant effects were investigated at different doses.Results： Water-soluble alkaloids from Eucommia male flowers could decrease the frequency of spontaneous activities,significantly increase sleeping ratio below the threshold dose,prolong the sleeping time and shorten the sleeping latency.Meanwhile,these alkaloids also could effectively reduce the eclamptic ratio of the mice and significantly prolong the convulsion latency.Conclusion： Water-soluble alkaloids of Eucommia male flowers have obviously sedative and hypnotic effects in mice and there is a synergistic effect between them and pentobarbital sodium.