猪血多肽缓解疲劳作用研究

Fatigue-alleviating Effect of Peptides from Porcine Blood

目的:研究猪血多肽缓解疲劳的作用。方法:将200只雄性昆明种小鼠分为1个对照组和3个实验组,对照组灌胃蒸馏水,实验组分别灌胃0.83、1.70、3.33g/kg猪血多肽,连续灌胃30d后,记录小鼠负重游泳时间和常压耐缺氧时间,检测样品对小鼠红细胞数(RBC)、血红蛋白含量(Hb)、肝糖原、肌糖原、尿素氮(BUN)、血乳酸(LA)指标的影响;再将32只雄性昆明种小鼠分为对照组和实验组,对照组灌胃蒸馏水,实验组灌胃1.70g/kg猪血多肽,连续灌胃30d后,检测样品对小鼠超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)、乳酸脱氢酶(LDA)、肌酸激酶(CK)指标的影响,并对骨骼肌和心肌作组织切片观察。结果:猪血多肽能显著延长小鼠负重游泳时间和常压耐缺氧时间;明显增加RBC、Hb含量,肝、肌糖原储备量及SOD、GSH-PX活性;降低BUN、MDA、NO含量和NOS、LDA、CK活性及LA曲线下面积;对运动后骨骼肌和心肌的损伤有保护作用。结论:猪血多肽具有明显的缓解疲劳作用,以1.70g/kg剂量效果最佳。其抗疲劳作用与增强抗氧化酶活性(以减轻长时间耐力运动导致自由基产生过多而对骨骼肌和心肌的损伤)和降低脑组织中NOS活性(以减少NO生成过量后中枢疲劳的发生)以及增强机体有氧代谢能力等作用有关。

Objective： To investigate the fatigue-alleviating effect of peptides from porcine blood（PPB）.Methods： Totally 200 male Kunming mice were randomly divided into one control group and three test groups.The mice from the control group were administered intragastrically with distilled water,and the mice from three test groups were administered intragastrically with PPB at doses of 0.83,1.70 g/kg and 3.33 g/kg for 30 consecutive days.At 30 min after the last administration,loaded swimming time and anti-hypoxia survival time of the mice were recorded.The fatigue indices including red blood cell number,hemoglobin content,hepatic glycogen,muscle glycogen,blood urea nitrogen（BUN）,blood lactate of the mice were assayed.Then,32 more male Kunming mice were divided into one control group and one test group.The control group and the test group were administered intragastrically with distilled water and PPB at the dose of 1.70 g/kg for 30 consecutive days,respectively.At 30 min after the last administration,the activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,nitric oxide synthase（NOS）,lactate dehydrogenase（LDH）,creatine kinase（CK）,and the contents of malondialdehyde（MDA） and nitric oxide（NO） were measured.The skeletal and cardiac muscles were subjected to histopathological examination.Results： PPB could significantly prolong the loaded swimming time and anti-hypoxia survival time of the mice,and obviously increase the number of red blood cells and the contents of hemoglobin,hepatic glycogen and muscle glycogen as well as the activities of SOD and GSH-Px,decrease the activities of NOS,LDH and CK,and the contents of BUN,MDA,NO and LA.PPB had obvious protective function against exercise-induced damage of skeletal and cardiac muscles.Conclusion： PPB has obvious fatigue-alleviating effect at the optimal dose of 1.70 g/kg.Its anti-fatigue effect is correlated to enhanced antioxidant enzyme activities（resulting in the alleviation of skeletal and cardiac muscle damages caused by excess free radicals generated after endurance exercise for a long time）,reduced occurrence of central fatigue following the production of excess NO as a result of lower activity of NOS and enhanced aerobic metabolism capacity in the body.