肺组织不同病变中M tp53、PCNA、S_(100)^+DC的异常表达及其临床病理学意义

The Clinicopathological Significance of Expression of Mtp53、PCNA and S 100 + DC Protein in Various Pulmonary Lesions

目的：探讨 ｐ５３ 蛋白（ Ｍｔｐ５３）、增殖细胞核抗原（ Ｐ Ｃ Ｎ Ａ）、 Ｓ１００ 阳性树突状细胞（ Ｓ１００＋ Ｄ Ｃ）在肺癌组织不同病变中的异常表达以及它们之间的调控关系。方法：用免疫组化技术对１１４ 例肺癌组织，１５ 例肺良性肿瘤，１２ 例肺部炎症中的ｐ５３、 Ｐ Ｃ Ｎ Ａ 及 Ｓ１００＋ Ｄ Ｃ蛋白的异常表达进行半定量研究。结果：ｐ５３ 蛋白仅在肺癌中表达，且其表达水平与肺癌的组织学类型、细胞分化程度和淋巴结转移无关。 Ｐ Ｃ Ｎ Ａ 在肺癌、良性肿瘤及炎性组织中均有不同程度的表达，癌组织中的表达（５７．０％ ）显著高于良性肿瘤（２０．０％ ）、肺部炎症（１６．７％ ）和正常组织（７．１％ ）。 Ｓ１００＋ Ｄ Ｃ表达在肺癌仅为２３．７％ （小细胞肺癌中无表达），显著低于肺部炎症（６６．７％ ）和正常组织（５０．０％ ）。在癌性组织中其表达水平与细胞分化程度和淋巴结转移情况相关。ｐ５３ 与 Ｐ Ｃ Ｎ Ａ 蛋白之间存在正调控关系。结论：（１）ｐ５３、 Ｐ Ｃ Ｎ Ａ、 Ｓ１００ ＋ Ｄ Ｃ的异常参与肺癌的发生、发展。（２）肺部非癌性病变组织中无 ｐ５３ 基因突变，但细胞仍处于低增殖高免疫活性状态。（３）肺癌组织中不仅有 Ｍｔｐ５３、 Ｐ Ｃ Ｎ ?

Objective: To investigate the roles of p53, PCNA and S 100 + DC protein alterations in various pulmonary lesions including pulmonary carcinomas, non malignant tumors, inflammations, and the relations between p53 and PCNA, S 100 + DC. Methods: Immunohistochemistry was employed to detect the expression of p53, PCNA and S 100 + DC protein in pulmonary carcinomas(114), inflammations (12) and non malignant tumors(15). 14 cases of normal lung tissues were used for control. Results: p53 protein exsisted only in pulmonary carcinomatous tissues, its expression wasn't related to histological grades, types and lymph node status (P>0.05). PCNA protein was detected in all pulmonary lesions. Overexpression in carcinomas (57.0%) was greatly higher than in non malignant tumors (20.0%), inflammations (16.7% and normal tissues (7.1%). In contrast,S 100 + was only expressed 23.7% in carcinomas (there wasn't in SCLCS), which was lower than 66.7% in inflammations and 50.0% in normal tissues. And it was correlated with histological grades, types and lymph node metastasis. Besides,ther was positive regularity between p53 and PCNA. Conclusions: (1)Mtp53 protein doesn't exist in noncarcinomatous lesions of the lung, but cells consistantly proliferate with a higher immunocompetency. (2) In carcinomatous lesions there are overexpression of mutant p53 genes, PCNA, these patients decline in immunoactivity. (3) It may exist positive regularity between p53 and PCNA