大鼠在体肠回流法研究三七总皂苷壳聚糖微球的吸收行为

Absorption behavior of saponins of Panax Notoginseng chitosan microsphere by in situ circulation method in rat's intestine

目的:研究三七总皂苷壳聚糖微球在大鼠小肠的吸收行为。方法:采用乳化交联法制备三七总皂苷壳聚糖微球。大鼠在体肠回流法研究微球在大鼠小肠的吸收行为。结果:当微球中药物浓度从0.213 8g.L-1逐渐增至0.855 4g.L-1时,累积吸收量从(0.064±0.003)mg.cm-2增至(0.391±0.017)mg.cm-2,但当浓度继续增至1.069 2g.L-1时,累积吸收量缓慢增至(0.402±0.014)mg.cm-2,呈现饱和现象。加入空白微球,累积吸收量明显减少(P<0.01);降低Na+浓度,加入P-糖蛋白抑制剂(盐酸维拉帕米)和能量抑制剂(2,4-二硝基苯酚,DNP),对累积吸收量没有显著影响。结论:三七总皂苷壳聚糖微球在大鼠小肠的吸收是以被动扩散的吸收方式为主,以其他吸收方式为辅。

OBJECTIVE To investigate the absorption behavior of saponins of panax notoginseng （PNS）chitosan microspheres in the small intestine of rats. METHODS PNS chitosan microspheres were prepared by emulsion-chemical crosslinking technique. In situ circulation method in rat＇s intestine was utilized. RESULTS As the concentration of PNS increased from 0. 2138 g.L ^-1 to 0. 8554 g·L^-1 , the accumulated absorption increased correspondingly from （0. 0642 ±0.0026 ） mg·cm^-1 to （0. 3906 ±0.0174） mg·cm^- 2. But when the concentration of PNS increased to 1. 0692 g· L^- 1 , the absorption amount in- creased steadily to （0. 4019 ± 0. 0138） mg·cm^-2, which showed a saturated absorption. Adding blank microspheres had predominant influence on absorption （P〈0. 01） ; decreasing the concentration of Na ＋ , adding P-glycoprotein inhibitor （verapamil hydrochloride） and the energy inhibitor （DNP） had no influence on absorption. CONCLUSION The main absorption of sapo- nins of PNS chitosan microspheres in the small intestine of rats may be transported through passive diffused absorption pattern, and it has other diffused absorption patterns.