牛附睾和附睾液分析与牛附睾尾精子在模拟附睾液中存活试验

Medium-term Survival of Bovine Caudal Epididymal Sperm under Conditions Based on Epididymal Fluid Characteristics

【目的】新鲜精液在离开生殖腺后存活时间很短。相关研究表明副睾液为精子的存活提供了特殊的环境。试验探讨和研究牛附睾精子在体外模拟附睾液中的存活试验的各因素的影响。【方法】试验对公牛附睾头部和尾部的摩尔渗透压和蛋白质浓度进行了分析,牛附睾尾部精子在不同蛋白浓度的CEP-2溶液中,在4℃条件下孵育120 h。每24 h做一次精子活力检测。【结果】5 d后精子活力仍然超过60%。附睾头、尾部的渗透压分别为(287.0±13.7)mOsm和(310.8±17.0)mOsm。蛋白浓度分别为(37.43±12.55)mg/mL和(50.58±11.08)mg/mL。【结论】附睾尾精子在蛋白浓度为40 mg/mL,渗透压为345 mOsm时,精子存活率最高。

[ Objective] Fresh semen has a very limited life span after it leaves the gonad. The relative research showed that the epididymal plasma provides a special environment to spermatozoa for survival. In this study, the osmolarity and protein concentration of caput and caudal bull epididymis were analyzed. [ Method ] Bovine caudal epididymal sperm was incubated in different protein and sorbitol combinations of CEP - 2 diluents at 4℃ for 120h and sperm viability was assessed. [ Result] Under these conditions, sperm viability was still more than 60 % after 5 days. The osmolarity of caput and caudal epididymis were （287.0±13.7）mOsm and （310.8±17.0）mOsm respectively. The protein concentration of caput and caudal epididymis were （37.43±12.55）mg/ml and （50.58±11.08）mg/mL respectively. This may have an application in the animal production industry. [Conclusion]The highest viability of fresh extracted caudal epididymal sperm was in the modified CEP - 2 diluent with 40mg/ml protein concentration and 345 mOsm.