新孢子虫病rELISA抗体检测试剂盒的应用

Application of an Indirect rELISA Kit for the Detection of Antibody against Neospora Caninum

【目的】研发一种成熟稳定的新孢子虫病rELISA抗体检测试剂盒。【方法】在新孢子虫病rELISA检测方法建立的基础上,组装检测新孢子虫病的rELISA抗体检测试剂盒,对试剂盒的敏感性、保存期、特异性和重复性进行评价,并与两种商品化试剂盒进行对比试验,应用自制的试剂盒检测来自新疆各地州新孢子虫疑似病例区血清,共1 329份。【结果】成功组装了新孢子虫病rELISA抗体检测试剂盒,且该试剂盒敏感性高、保存期长、特异性强、重复性较好。与两种进口商品化诊断试剂盒比较,其符合率均在90%以上。在1 329样品血清中157份是阳性,其平均阳性率为11.8%。【结论】该试剂盒可以取代商品化试剂盒进行新孢子虫病诊断,为新孢子虫病的防治工作提供技术和产品支撑。

[Objective]To develop a proven and stable indirect rELISA kit for detection of antibody against Neospora caninum. [Method] An indirect rELISA kit for the detection of antibody against Neospora caninum was developed based on the optimal reaction conditions which were selected previously. The developed kit was evaluated for sensitivity, retention period, specificity and reproducibility, and compared with two kinds of commercial ELISA kits. Blood samples were collected from 1 329 animals in several suspected areas of Xinjiang Uyghur Autonomous Region and tested them for antibodies against Neospora caninum by the kit. [ Result ] An indirect rELISA kit for detection of antibody against Neopora caninum was developed. The kit has long retention period, high specificity, sensitivity, and reproducibility. The agreements between the developed kit and two kinds of commercial ELISA kits were more than 90%. One hundred and fifty seven serum samples were positive among 1 329 samples, and the seroprevalence was 11.8 %. [ Conclusion] The kit can be a substitute for commercial ELISA kits to test antibodies against Neospora caninum, and a technique for to prevention of the Neosporosis is provided.