沙门菌、志贺菌和金葡菌复合增菌培养基研制

Enrichment broth for simultaneous culture of Salmonella,Shigellae,and Staphylococcus aureu

目的研制用于沙门菌、志贺菌和金黄色葡萄球菌同时增菌的培养基。方法根据微生物营养素需求,筛选可添加成分进行单因素比较试验,确定出培养基合适的组分,采用平板菌落计数法和多重PCR法验证培养基增菌效果。结果成功研制出能够用于沙门菌、志贺菌和金黄色葡萄球菌复合增菌的培养基（3S）,当接种量约为1 CFU/mL,37℃培养12 h后,3种目标菌以相对一致的速度增殖,可达到107~108CFU/mL;应用多重PCR可同时扩增出目的条带。结论 3S可用于沙门菌、志贺菌和金黄色葡萄球菌复合增殖培养,配制方便、增菌快速、节约成本和时间,具有良好的市场前景。

Objective To develop an enrichment medium for simultaneous culture of Salmonella,Shigellae and Staphylococcus aureu.Methods According to the six micro-nutrient requirements,the different components were compared and the components for the enrichment medium were selected.The effect of the enrichment broth was validated with plate count and multiplex PCR methods.Results An enrichment medium（3S） was obtained which could be used to culture Salmonella,Shigellae and Staphylococcus aureu concurrently.When 10 CFU per one milliliter of Salmonella,Shigellae and Staphylococcus aureu were simultaneously added to the 3S and incubated for 12hr at 37℃,the bacteria multiplied at the same speed,and reached 107-108 CFU per one milliliter.Multiplex PCR could be used to produced specific amplicons of expected sizes.Conclusion 3S can be used to enrich Salmonella,Shigellae and Staphylococcus aureu simultaneously and can be applied in rapid detection of the bacteria conveniently.