摘要
通过对不同外植体的筛选和培养条件的优化,建立了甜高粱再生体系。结果表明,以茎尖和茎为外植体的培养可获得甜高粱再生苗,但分化率较低,褐化较为严重;而以叶和根为外植体的培养不能获得甜高粱再生苗;成熟胚作外植体分化率高达98.7%,且未发生褐化。
Through different explants screening,the optimization of culture conditions to build sweet sorghum regeneration system was conducted.The results showed that shoot tips and stems were cultured as explants and renewable sweet sorghum seedlings could be obtained,but with the lower rate of differentiation and serious browning.Leaf and root were cultured as explants but failed to obtain renewable sweet sorghum seedlings.The use of renewable mature embryos could obtained the differentiation rate of 98.7% and without the browning phenomenon.
出处
《作物杂志》
CAS
CSCD
北大核心
2011年第3期24-28,共5页
Crops
基金
内蒙古自治区重大科技专项
内蒙古自然科学基金重大项目(2009ZD03)
教育部春晖计划项目(Z2007-1-01017)基金资助
关键词
甜高粱
成熟胚
茎尖培养
再生
Sweet sorghum
Mature embryo
Shoot tip culture
Regeneration
