海洋放线菌Streptomyces sp.次级代谢产物的研究

Study on the secondary metabolites from a marine-derived actinomycete Streptomyces sp.

目的系统研究海洋放线菌Streptomyces sp.的次级代谢产物。方法采用硅胶开放柱色谱、SephadexLH-20凝胶柱色谱以及高效液相色谱等分离方法对Streptomyces sp.的次级代谢产物进行分离、纯化;根据理化性质和波谱学分析对所得单体化合物进行结构鉴定。结果与结论从海洋放线菌Streptomyces sp.发酵物的乙酸乙酯萃取物中分离得到12个已知化合物,分别鉴定为3-苄基吡咯并哌嗪-25,-二酮(1)、3-苄基-8-羟基吡咯并哌嗪-25,-二酮(2)3、-对羟苄基-8-羟基吡咯并哌嗪-25,-二酮(3)、3-([1H-吲哚-3'-基)甲基]吡咯并哌嗪-25,-二酮(4)3、-异丁基-6-异丙基哌嗪-2,5-二酮(5)、3-异丙基吡咯并哌嗪-25,-二酮(6)、二吡咯并哌嗪-25,-二酮(7)、N-苯乙基乙酰胺(8)、4'7,-二羟基异黄酮(9)4、'5,7,-三羟基异黄酮(10)4、',7-二羟基二氢黄酮(11)、吡咯-2-甲酸(12)。以上化合物均为首次从该链霉菌属菌株中分离得到。

The secondary metabolites of marine-derived actinomycete Streptomyces sp.were systematically studied.The ethyl acetate extract of the fermentation broth was separated by modern chromatographic methods,such as silica gel column chromatography,Sephadex LH-20,TLC,preparative reversed-phase HPLC,and so on.The structures of the 12 compounds firstly isolated from the microorganism were elucidated as 3-benzyl-pyrrolopiperazine-2,5-dione（1）,3-benzyl-8-hydroxyl-pyrrolopiperazine-2,5-dione（2）,3-p-hydroxylbenzyl-8-hydroxyl-pyrrolopiperazine-2,5-dione（3）,3-（（1H-indol-3′-yl）methyl）-pyrrolopiperazine-2,5-dione（4）,3-isobutyl-6-isopropylpiperazine-2,5-dione（5）,3-isopropyl-pyrrolopiperazine-2,5-dione（6）,dipyrrolopiperazine-2,5-dione（7）,N-phenylethylacetamide（8）,4′,7-dihydroxyisoflavone（9）,4′,5,7-trihydroxyisoflavone（10）,4′,7-dihydroxyflavanone（11） and 2-pyrrolecarboxylic acid（12）,on the basis of their spectroscopic and physicochemical properties.