不同pH对甘草种子萌发及幼苗生长的影响

Study on the Effect of pH on Seed Germination and Seedling Growth of Glycyrrhiza uralensis

[目的]研究甘草种子萌发和幼苗生长的最适pH,优化甘草中药材产业化栽培技术。[方法]用不同pH溶液(pH3,5,6,7,8,9,10,11)处理甘草种子7 d,分别计算其发芽率、发芽势、发芽指数,并测定胚根胚芽长度及整株幼苗鲜重;采用水培法,以不同pH的营养液(pH为5,6,7,8,9)培养甘草幼苗,30 d后测定其生物量、药用成分,蛋白质,氨基酸,可溶性总糖,淀粉,丙二醛含量以及硝酸还原酶和过氧化物酶活性等指标。[结果]pH为6时甘草种子的发芽率最高、出苗整齐,且发芽后胚芽长度及幼苗鲜重均有最大值;溶液培养pH为6时,甘草生物量、甘草酸、蛋白质、淀粉等含量最高,同时其硝酸还原酶和过氧化物酶活性也最大。[结论]pH6时最利于甘草种子的萌发,幼苗生长及药用成分的积累。

[Object] To investigate the optimum pH for the seed germination and seedling growth of Glycyrrhiza uralensis,and optimize its industrialized cultivation technology.[Methods] The seeds of Glycyrrhiza uralensis were soaked in solutions with different pH for 7 d（pH 3,5,6,7,8,9,10,11）,and the germination rate,germination energy,germination index,the length of radicle and plumule,fresh weight of seedlings were determined;then the seedlings of Glycyrrhiza uralensiswere cultivated in solutions with different pH（5,6,7,8,9）for 30 d,and the biomass,contents of medicinal compositions,protein,amino acids,soluble total sugar,starch and MDA,the activities of nitrate reductase and peroxidase etc.indexes were determined.[Result] The germination rate of Glycyrrhiza uralensis seeds,the length of radicle and plumule all achieved the highest level when pH was 6,and the seedlings came out evenly;besides,the biomass,the contents of medicinal compositions,protein,amino acids,soluble total sugar,starch and MDA as well as the activities of nitrate reductase and peroxidase all reached the maximum value with pH of 6.[Conclusion] The optimum pH for the germination of Glycyrrhiza uralensis seed,the growth of seedlings and the accumulation of medicinal ingredients was 6 in this study.