浙贝母花及花粉中8种E族维生素的高效液相色谱—荧光分析

Determination of E Vitamins in the Flowers and Pollens of Fritillaria thunbergii Miq. by HPLC-FLD

[目的]分析浙贝母花及其花粉中E族维生素的组成和含量,为浙贝母花资源的开发提供一定的科学依据。[方法]采用高效液相色谱—荧光分析法(HPLC-FLD)检测E族维生素的成分,检测条件如下:Agilent Zorbax RX-SIL(4.6 mm×150 mm,5μm)色谱柱,4.6mm×10 mm保护柱,含0.2%异丙醇和0.8%冰乙酸的正己烷溶液为流动相(1∶4,V/V),流速为1.0 ml/min进行洗脱,柱温25℃,荧光最大激发波长292 nm,最大发射波长328 nm。[结果]首次建立了可同时测定8种维生素E成分的高效液相色谱—荧光分析法,8种成分在检测范围内均具有良好的线性关系,R2>0.999 0,加样回收率大于98%。结果表明浙贝母花中共含有7种E族维生素成分,其中D-α-生育三烯酚含量最高,达181.606 mg/kg,而浙贝母花花粉中E族维生素成分的含量则偏低。[结论]浙贝母花和花粉中均含有较多的E族维生素成分,其中浙贝母花中所含的种类和数量多于花粉,可作为新的药食两用资源进一步开发。同时,本研究建立的HPLC-FLD方法快速高效,适于分析和测定E族维生素的含量。

[Objective] To analyze and determine the chemical structures and contents of E vitamins in flowers and pollens of F.thunbergii Miq.,so as to provide scientific basis for its further development.[Methods] High-performance liquid chromatography-florescence detection（HPLC-FLD） was first established to determine the compositions of E vitamins.The detection conditions were as follows：Agilent Zorbax RX-SIL column（4.6 mm×150 mm,5 μm） and guard column（4.6 mm×10 mm,5 μm）,mobile phase of hexane-0.2% isopropanol-0.8% acetic acid（1∶4,V/V）,column temperature of 25 ℃,flow rate of 1.0 ml/min,the maximum excitation wavelength of 292 nm and the maximum emission wavelength was 328 nm.[Results]8 kinds of vitamins were determined simultaneously for the first time,which were in good linear relationship within the detection range,R2〉0.999 0 and recovery rate exceeded 98%.There were 7 kinds of E vitamins in the flower of F.thunbergii Miq.,among which D-α-tocotrienols was the highest that reached 181.606 mg/kg,however the contents of E vitamins in the pollens of F.thunbergii Miq.were relatively lower.[Conclusion] There are multiple kinds of E vitamins in the flowers and pollens of F.thunbergii Miq.,comparatively speaking,the flower of F.thunbergii Miq.is richer in E vitamins,which deserves further research.Besides,HPLC-FLD is convenient and efficiency that can be used for determining the contents of E vitamins.