荷斯坦种公牛HSF1和HSBP1基因多态性分析

Analysis of Genetic Polymorphisms of HSF1 and HSBP1 Gene in Holstein Ox

[目的]研究荷斯坦种公牛HSF1和HSBP1基因多态性。[方法]通过DNA测序技术对牛HSF1和HSBP1基因进行SNPs位点扫描,利用CRS-PCR和PCR-RFLP方法对4个单核苷酸多态性(SNPs)进行基因型分型,分析162头荷斯坦种公牛HSF1和HSBP1基因的多态性。[结果]扫描结果表明,在HSF1基因1451(G/T)处发现1个新SNP。在HSBP1基因第2内含子上发现3个新SNPs,分别为324(G/C)、589(C/T)和651(C/G)。多态性分析结果表明,HSF1基因1451(G/T)位点的SNP的AA基因型频率最高,优势等位基因为A,而HSBP1基因3个SNP频率最高的基因型分别为AB、AA和BB,优势等位基因589(C/T)为A,而324(G/C)和651(C/G)均为B。χ2适合性检验表明,HSF1基因1451(G/T)位点在荷斯坦种公牛群体中已达到Hardy-Weinberg平衡状态(P>0.05),而HSBP1基因324(G/C)、589(C/T)和651(C/G)位点的突变在荷斯坦种公牛群体中均未达到Hardy-Weinberg平衡状态(P<0.05)。[结论]该研究可为HSF1和HSBP1基因的功能研究提供试验依据。

[Objective] The aim was to study the genetic polymorphisms of HSF1 and HSBP1 gene in Holstein ox.[Method] DNA direct sequencing was used for SNPs loci scanning,and CRS-PCR and PCR-RFLP method were used for genotyping of four SNPs in 162 Holstein ox,so as to analyze the polymorphism of HSF1 and HSBP1 gene.[Result] The scanning results showed that a novel SNP was discovered at 1451（G/T）in HSF1 gene and three novel SNPs of 324（G/C）,589（C/T）,651（C/G） were discovered in the second intron of HSBP1 gene.The polymorphism of HSF1 and HSBP1 gene analysis results showed that,for HSF1,the frequency of the AA genotype was higher and A allele was the dominant allele.While for HSBF1,AB,AA and BB appeared more frequently,dominant allele at 589（C/T） was A,and that at 324（G/C） and 651（C/G） was B.Chi-square test indicated that 1451（G/T） polymorphic site in the HSF1 gene and 589（C/T） polymorphic site in HSBP1 gene were met Hardy-Weinberg equilibrium in Holstein（P〉0.05）,while 324（G/C） and 651（C/G） polymorphic sites in the HSBP1 gene were not meet Hardy-Weinberg equilibrium（P〈0.05）.[Conclusion] The study can provide trial basis for deeply researching the function of HSF1 and HSBP1 gene.