大鼠背侧海马生长抑素在青霉素致癎中的作用

Effect of Somatostatin in Rat Dorsal Hippocampus on Penicilline Induced Epilepsy

目的 观察大鼠海马内生长抑素在癫中的作用。方法 14肽生长抑素(SS14)或半胱胺微量注入背侧海马,在大鼠感觉运动皮质记录脑电功率,应用推挽灌流结合放免法测定青霉素致后背侧海马内生长抑素免疫反应物质(SLI)含量的变化。结果 致后0.5hSLI含量显著增加,可持续4h。SLI含量的上升程度与致后感觉运动区脑电相对功率增加呈正相关。单侧背海马内注入SS140.03nmol使大鼠感觉运动区出现棘尖波,行为上表现样活动。单侧背海马内注入SS耗竭剂半胱胺30μg减少SLI含量30%左右,原先致剂量青霉素所诱发的癫程度减轻。

To study the role of hippocampus somatostatin on seizure in rat. Methods Somatostatin 14 (SS*.14) and cysteamine were microinjected into unilateral dorsal hippocampus.The EEG spectrum power in the sensorimotor cortex was recorded.The contents of SS*.14 in the rat dorsal hippocampus were measured by puch pull perfusion and readioimmunoassay during penicilline induced epilepsy(PIE). Results The contents of SS*.14 like immunoreactivity (SLI) and the EEG relative power significantly increased after 0.5 h PIE.The enhancement maintained to 4 h.Furthermore,there is a positive correlation between the severity of seizure and contents of SLI.SS*.14 0.03 nmol induced EEG relative power markedly increased and epileptiform activities for several hours.After reducting 30% of SLI by microinjection of 30 μg cysteamine,the severity PIE was attenuated. Results The contents of SS*.14 like immunoreactivity (SLI) and the EEG relative power significantly increased after 0.5 h PIE.The enhancement maintained to 4 h.Furthermore,there is a positive correlation between the severity of seizure and contents of SLI.SS*.14 0.03 nmol induced EEG relative power markedly increased and epileptiform activities for several hours.After reducting 30% of SLI by microinjection of 30 μg cysteamine,the severity PIE was attenuated. Conclusions Somatostatin 14 in the rat dorsal hippocampus is involved in seizure.