醋酸洗必泰与茜素络合物显色反应的研究

STUDY OF THE REACTION BETWEEN CHLORHEXIDINE ACETATE AND ALIZARIN COMPLEXONE

目的： 通过醋酸洗必泰与茜素络合物的显色反应来测定洗必泰栓中醋酸洗必泰的含量。方法： 用７２１ 型分光光度计， 利用分光光度法测定洗必泰的药物含量。结果： 在乙醇—丙酮介质中醋酸洗必泰与茜素络合物发生显色反应， 反应产物的λｍ ａｘ＝ ５３５ｎｍ ， 表观摩尔吸光系数为１２７×１０３ Ｌ·ｍ ｏｌ－ １ ·ｃｍ － １， 相对标准偏差为０７％ （ｎ＝ ６）， 药物浓度在２０～１００ｍ ｇ· Ｌ－ １ 范围内呈线性关系。结论： 本方法操作简便， 条件易于控制， 灵敏度较高， 对药物的测定结果令人满意。

Objective:To determine the content of chlorhoxidine acetate through reaction between chlorhexidine acetate and alizarin complexone Methods:By spectropholometry method with 721 Results:The reaction between chlorhexidine acetate and alizarin complexone took place in ethanol-acetone The apparent molar obsorptivity was 1 27×10 3L mol -1 ·cm -1 at 535nm The beer's law was conformed to in the range of 20~100mg·L -1 of chlorhexidine acetate in suppository sotisfactoriyl Conclusion:The method is simple with easily-controlled reaction conditions and highly satisfactory