广州地区6493例女性生殖道人乳头状瘤病毒检测结果分析

Analysis of detection of human papillomavirus in 6493 cases in Guangzhou

目的了解广州地区妇女生殖道人乳头状瘤病毒感染状况和基因亚型分布。方法 2006年10月至2010年2月,采用核酸分子快速导流杂交基因芯片分型技术(HybriMax)对广州地区6493例女性进行人乳头状瘤病毒检测,并对HPVDNA亚型、感染率和年龄分布进行分析。结果 6493例女性中检出HPV阳性1947例,阳性率为29.99%。阳性感染者中,单一型感染最多,为1436例,占73.75%,其中高危单一型感染者1143例,占58.71%,低危单一型感染者293例,占15.05%;混合型感染者511例,占26.25%,主要为双重感染,占19.41%。15个与宫颈癌密切相关的HPV高危亚型阳性率为25.24%,6个低危亚型HPV感染阳性率为7.98%。排在前十位的亚型分别是HPV52(25.22%)、HPV58(14.20%)、HPV16(13.56%)、HPV6(9.76%)、HPV11(8.32%)、HPV53(7.70%)、HPV33(6.73%)、CP8304(6.68)、HPV68(6.63)、HPV18(6.52%)。在各年龄组中,小于20岁女性感染率最高,为52.5%,各年龄组HPV感染差异有统计学意义(χ2=17.926,P<0.05)。不同来源患者的HPV阳性率分别是30.66%,19.24%,28.24%,35.92%,差异有统计学意义(χ2=23.274,P<0.05)。结论广州地区女性有相当高比例的HPV感染,HPV52、58、16、6亚型是最主要的感染亚型,进行HPVDNA检测和分型对宫颈病变的防治具有重要意义。

Objective To evaluate prevalence of human papillomavirus （HPV） infection and subtypes character of the virus in the female lower genital tract in Guangzhou city. Methods Between Oct. 2006 and Feb. 2010,6493 women in Guangzhou were selected for HPV subtype analysis using flow-through hybridization and gene chip （HybriMax）. The overall HPV prevalence,infectious status of various subtypes and age distribution in the 6493 women were compared and analyzed. Results Among the 6493 patients, 1947 cases were infected with HPV, the overall HPV prevalence was 29. 99%. 1463 cases （73.75%）were single HPV type infection,the high risk single HPV was detected in 58.71% of the single HPV positive women,the low risk HPV was detected in 15.05% of the single HPV type infection. The total multiple infection rate was 26. 25% and two types co-infection were the most commonsituation. Out of the 21subtypes of HPV, 15 were associated with high risk types of HPV, with a positive rate of 25. 24%, the low risk HPV was detected in 7.98% of the HPV positive women. The 10 most common subtypes were （ in descending sequences ） ： HPV52 （ 25.22% ）, HPV58 （ 14. 20% ）, HPV16 （13.56%）,HPV6（9.76% ）, HPV11 （ 8. 32% ）, HPV53 （7.70%）, HPV33 （6. 73% ）, CP8304 （6. 68 ）, HPV68 （6. 63 ）, HPV18（6. 52% ）. The highest HPV prevalence was found in women at the age of 〈20 years,there was significant differences among the age groups （x^2 = 17. 926 ,P 〈 0. 05 ）. The rate of different sources group were 30. 66%, 19. 24% ,28. 24%, 35.92% respectively and had significant differences （x^2 = 23.274,P 〈 0. 05 ）. Conclusion HybriMax detects a high prevalence of HPV in the women in Guangzhou,and HPV52, HPV58, HPV16, HPV6 are the most frequent subtype. Test and subtype analysis of HPV play an important role in prevention and management of cervical neoplasia.