蜂胶对炭疽杆菌的抑菌活性

Antibacterial activity of propolis against Bacillus anthracis

目的了解产自河南省的蜂胶对炭疽杆菌的抑菌作用。方法采用琼脂平板扩散法,将实验分为pH5.5～8.5不同组(蜂胶浓度31%),以及在pH5.5下设置10个不同浓度组,即将31%蜂胶溶液倍比稀释至0.06%(1∶512)及空白组(0),每组3次重复,每张滤纸片(?6mm)滴加蜂胶溶液7μl,记录蜂胶的抑菌效果。结果河南省蜂胶在pH5.5、6.0、6.5、7.0、7.5、8.0、8.5平板上的抑菌环直径依次为(19.16±0.29)、(17.67±0.29)、(14.67±0.58)、(13.67±0.29)、(14.00±0.00)、(13.33±1.15)、(12.00±0.00)mm,蜂胶浓度分别为15.50%、7.75%、3.88%、1.94%、0.97%、0.48%、0.24%、0.12%、0.06%和0(空白组)时,抑菌环直径依次为(19.50±1.80)、(19.67±1.04)、(17.75±0.35)、(15.67±0.58)、(14.00±2.29)、(13.17±1.04)、(11.83±1.53)、(10.83±1.26)、(9.00±0.00)和(0.00±0.00)mm。结论河南省蜂胶对炭疽杆菌有明显的抑菌活性,对炭疽杆菌的抑菌活性随pH值增加或浓度降低而减小。

Objective To determine the antibacterial activity of propolis from Henan province against Bacillus anthracis. Methods The agar diffusion method was used to test for antibacterial activity. Antibacterial effects were measured after exposure to seven formulations of 31% propolis at pH ranging from 5.5-8.5（5.5,6.0,6.5,7.0,7.5,8.0,8.5） . Additionally,ten dilutions of propolis from 31% to 0.06%（1∶512） ,including a 0 control,at pH 5.5 and a dose of 7 μl were examined in triplicate. Results The diameters（mm） of the zones of inhibition in each pH group,5.5,6.0,6.5,7.0,7.5,8.0 and 8.5,were as follows：19.16±0.29,17.67±0.29,14.67±0.58,13.67±0.29,14.00±0.00,13.33±1.15 and 12.00±0.00 mm,respectively. The diameters in each concentration group,15.50%,7.75%,3.88%,1.94%,0.97%,0.48%,0.24%,0.12% and 0.06%,and the control group were：19.50±1.80,19.67±1.04,17.75±0.35,15.67±0.58,14.00±2.29,13.17±1.04,11.83±1.53,10.83±1.26,9.00±0.00 and 0.00±0.00 mm,respectively. Conclusion Propolis from Henan province had antibacterial activity against B. anthracis,which declined with increasing pH or decreasing concentration.